Clonal Cell Derivation
Clonal Cell Derivation - Related Videos
10:32
Enumeration of Neural Stem Cells Using Clonal Assays
0 Views •
Cited by 5 •
2016
Neural stem cells (NSCs) refer to cells which can self-renew and differentiate into the three neural lineages. Here, we describe a protocol to determine NSC frequency in a given cell population using neurosphere formation and differentiation under clonal conditions.
12:06
Isolation of CD133+ Liver Stem Cells for Clonal Expansion
0 Views •
Cited by 22 •
2011
Here we describe the isolation of CD133 expressing liver stem cells and cancer stem cells from whole murine liver, a process that requires tissue digestion, cell enrichment, and flow cytometry isolation. We include methods for advanced single cell isolation and clonal expansion.
01:22
T Cell Activation and Clonal Selection
0 Views •
2024
T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection. Naive T cells that have not yet encountered an antigen express two primary CD...
17:08
In vivo Clonal Tracking of Hematopoietic Stem and Progenitor Cells Marked by Five Fluorescent Proteins using Confocal and Multiphoton Microscopy
0 Views •
Cited by 11 •
2014
Combinatorial 5 fluorescent proteins marking of hematopoietic stem and progenitor cells allows in vivo clonal tracking via confocal and two-photon microscopy, providing insights into bone marrow hematopoietic architecture during regeneration. This method allows non-invasive fate mapping of spectrally-coded HSPCs-derived cells in intact tissues for extensive periods of time following transplantation.
06:40
Visualization of Chondrocyte Intercalation and Directional Proliferation via Zebrabow Clonal Cell Analysis in the Embryonic Meckel’s Cartilage
0 Views •
Cited by 10 •
2015
Cell organization of craniofacial bones has long been hypothesized but never directly visualized. Multi-spectral cell labeling and in vivo live imaging allows visualization of dynamic cell behavior in zebrafish lower jaw. Here, we detail the protocol to manipulate Zebrabow transgenic fish and directly observe cell intercalation and morphological changes of chondrocytes in the Meckel’s cartilage.
FAQs
A population descended from one progenitor has a shared cellular origin, which helps investigators distinguish cell-intrinsic traits from variation already present within a mixed population. This controlled origin can make comparisons between experimental groups more interpretable, particularly when researchers examine disease-associated changes, gene function, or treatment responses.
Culture conditions do more than support survival and proliferation: they can also select for unintended variants. As a result, a colony may change in ways that are not part of the original biological question. Monitoring genetic stability and other defining characteristics helps reveal whether the expanded population remains suitable for controlled investigation.
These approaches provide different ways to isolate an individual starting cell. Limiting dilution separates cells during culture setup, single-cell sorting isolates a cell through a sorting procedure, and colony picking selects a colony after growth. Each approach supports the same critical requirement: linking the resulting population back to a single progenitor.
The workflow begins by isolating a single cell, then placing it under culture conditions that support survival and proliferation. Once a colony develops, researchers screen it for identity, phenotype, and genetic stability. These checks connect the colony to its intended source and determine whether it provides a reliable population for downstream biological experiments.
Identity, phenotype, and genetic stability are central screening criteria. Identity confirms that the colony represents the intended cell type, phenotype assesses its observable biological characteristics, and genetic-stability testing helps identify changes acquired during culture. Reviewing all three areas provides a stronger basis for deciding whether the population is appropriate for further study.
They are useful when investigators need experimental consistency while studying disease-associated changes, evaluating gene function, or testing treatments. A shared cellular origin reduces population-level variation that could obscure interpretation. The resulting lines therefore support more controlled comparisons, although their characteristics still require validation because culture can select unintended variants.