Clonal Expansion
Clonal Expansion - Related Videos
12:06
Isolation of CD133+ Liver Stem Cells for Clonal Expansion
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Cited by 22 •
2011
Here we describe the isolation of CD133 expressing liver stem cells and cancer stem cells from whole murine liver, a process that requires tissue digestion, cell enrichment, and flow cytometry isolation. We include methods for advanced single cell isolation and clonal expansion.
10:32
Enumeration of Neural Stem Cells Using Clonal Assays
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Cited by 5 •
2016
Neural stem cells (NSCs) refer to cells which can self-renew and differentiate into the three neural lineages. Here, we describe a protocol to determine NSC frequency in a given cell population using neurosphere formation and differentiation under clonal conditions.
09:42
Analysis of Thermal Expansion via Dilatometry
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2023
Source: J. Jacob Chavez, Ryan T. Davis, and Taylor D. Sparks, Department of Materials Science and Engineering, The University of Utah, Salt Lake City, UT Thermal expansion is extremely important when considering which materials will be used in systems that experience fluctuations in temperature. A high or low thermal expansion in a material may or may not be desirable, depending on the application. For instance, in a common liquid thermometer, a material with a high thermal expansion would be...
10:15
Isolation and Culture Expansion of Tumor-specific Endothelial Cells
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Cited by 20 •
2015
We report a reliable method to isolate and culture primary tumor-specific endothelial cells from genetically engineered mouse models.
06:40
Visualization of Chondrocyte Intercalation and Directional Proliferation via Zebrabow Clonal Cell Analysis in the Embryonic Meckel’s Cartilage
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Cited by 10 •
2015
Cell organization of craniofacial bones has long been hypothesized but never directly visualized. Multi-spectral cell labeling and in vivo live imaging allows visualization of dynamic cell behavior in zebrafish lower jaw. Here, we detail the protocol to manipulate Zebrabow transgenic fish and directly observe cell intercalation and morphological changes of chondrocytes in the Meckel’s cartilage.
FAQs
Clonal expansion is controlled by signals that regulate cell-cycle entry. A stimulus can move a previously rare or inactive cell into repeated mitotic divisions, increasing the number of cells derived from that origin. Because regulation determines when proliferation begins and how the population develops, studying these signals helps connect external activation with changes in cell number and function.
In immune responses, antigen recognition can select a rare lymphocyte for expansion. The activated cell then produces many related descendants, some of which differentiate, linking recognition of a specific antigen with a larger population of functionally specialized cells. This example shows why expansion is biologically useful: it amplifies a response that begins with very few cells.
Proliferation increases the number of descendants produced from an original cell, whereas differentiation changes the properties or roles of those descendants. These processes can occur in sequence or alongside one another, as illustrated by antigen-stimulated lymphocytes that multiply and then differentiate. Separating the two concepts helps explain both population growth and the development of specialized cellular functions.
Regulation determines whether cell proliferation occurs in response to an appropriate stimulus and remains connected to biological function. When expansion becomes abnormal, the resulting growth can contribute to cancer and other diseases. Examining the signals and cell-cycle controls involved therefore helps researchers relate ordinary tissue or immune development to pathological cellular proliferation.
Clonal expansion helps explain several forms of population growth, including immune responses, tissue growth, and microbial population development. It also provides a framework for tracing cellular lineages because descendants retain a shared origin. Comparing these settings shows how the same basic pattern of increasing related cells can support defense, development, maintenance, or population establishment.
Because expanded cells descend from a common originating cell, their proliferation can reveal how cellular lineages arise and become organized. Researchers can use this biological framework to connect a starting cell with later populations that share an origin or function. In immune and tissue contexts, that connection helps explain how specific cellular groups develop from relatively limited starting populations.
Clonal expansion provides a way to examine how altered control of proliferation can produce an abnormally growing cellular population. Its relevance to cancer comes from the same principles that govern normal growth: signals influence cell-cycle entry, repeated divisions increase cell number, and descendants form a related population. Studying these relationships helps connect cellular regulation with disease development.