Coc Isolation
Coc Isolation - Related Videos
04:33
3D Culture of Porcine COCs: A Procedure to Encapsulate Cumulus Oocyte Complexes in Fibrin-alginate Polymer Hydrogel Beads
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2025
This video describes the protocols for encapsulating porcine cumulus-oocyte complexes (COCs) in fibrin-alginate beads to establish a 3D culture. This in-vitro maturation system maintains normal cell-to-cell communication, ensuring oocyte maturation and retaining viability.
03:02
Porcine Cumulus Oocyte Complex Isolation: A Technique to Isolate Cumulus Oocyte Complex from Porcine Ovarian Follicle for In Vitro Fertilization
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2025
This video describes the technique to isolate the cumulus-oocyte complex or COC from porcine ovaries. The isolated intact COC can be used to carry out the in vitro fertilization - the assisted reproductive technique for developing fertility treatments.
14:01
Isolation and Derivation of Mouse Embryonic Germinal Cells
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Cited by 4 •
2009
The ability of embryonic germinal cells to differentiate into primordial germinal cells during early development stages is a perfect model to address our hypothesis about cancer and infertility. This protocol shows how to isolate primordial germinal cells from developing gonads in 10.5-11.5 days post coitum mouse embryos.
08:09
Vasodilation of Isolated Vessels and the Isolation of the Extracellular Matrix of Tight-skin Mice
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Cited by 1 •
2017
We describe the isolation of cardiac extracellular matrix from C57Bl/6J control mice, tight-skin mice, and tight-skin mice treated with the IRF5 inhibitory peptide. We also describe the vasodilation studies on the isolated vessels from C57Bl/6J, tight-skin mice and tight-skin mice treated with the IRF5 inhibitory peptide.
07:12
Neutrophil Isolation Protocol
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Cited by 173 •
2008
Neutrophils are among the first cells to arrive on the site of inflammatory immune response, and their functions and mechanisms have been studied extensively in vitro. We demonstrate a standard density gradient separation method to isolate human neutrophils from whole blood using commercially available separation media.
FAQs
Retaining the cumulus layer preserves the cellular context surrounding the oocyte. These cells support oocyte development and provide a system for examining signaling between cumulus cells and the oocyte. Maintaining that relationship is especially relevant when researchers evaluate oocyte competence, reproductive toxicology, or developmental processes that may be altered when the surrounding cells are absent.
The isolated complexes can proceed to in vitro maturation, fertilization, or embryo culture, allowing investigators to follow outcomes beyond recovery. These downstream stages provide experimental settings for examining whether collected oocytes develop appropriately and for relating the original sample to reproductive or early developmental performance. Consistent isolation helps make comparisons between samples more reproducible.
Microscopic selection relies on the characteristic morphology of a cell-enclosed oocyte. After follicular material is released, researchers identify complexes that retain their surrounding cumulus cells, collect them, and wash them. This morphology-based handling helps distinguish the desired sample from other recovered material and supports collection of viable, representative complexes for subsequent experiments.
Recovery generally begins by releasing follicular contents through follicular aspiration or by dissecting the ovarian follicles. The recovered material is then examined microscopically to locate cell-enclosed oocytes. Identified complexes are collected and washed before being assigned to later procedures such as maturation, fertilization, or embryo culture, depending on the study design.
Researchers choose this approach when they need to study oocytes together with their supporting cumulus cells. It is useful for reproductive biology investigations, analyses of cumulus cell signaling, reproductive toxicology studies, and experiments tracking early development. The method also supplies material for in vitro maturation, fertilization, and embryo culture workflows.
Consistent isolation improves the likelihood that collected samples are both viable and representative. Differences in recovery, microscopic selection, or washing can change the material entering later experiments, making results harder to compare. Standardized handling therefore supports reproducibility when researchers assess oocyte competence, signaling, toxicological effects, or developmental outcomes across experimental groups.