Colony Expansion
Colony Expansion - Related Videos
12:17
Isolation and Large Scale Expansion of Adult Human Endothelial Colony Forming Progenitor Cells
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Cited by 38 •
2009
Endothelial colony forming progenitor cells (ECFCs) are a promising tool to study vascular homeostasis and repair.1,2 This paper introduces a novel animal-serum free method for isolation and expansion of ECFC from heparinised adult human peripheral blood with pooled human platelet lysate (pHPL) diminishing the risk of anti-bovine immunisation.
16:04
Isolation and Animal Serum Free Expansion of Human Umbilical Cord Derived Mesenchymal Stromal Cells (MSCs) and Endothelial Colony Forming Progenitor Cells (ECFCs)
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Cited by 66 •
2009
This protocol describes the isolation and subsequent expansion of mesenchymal stromal cells and endothelial colony forming cells without the use of animal serum to generate autologous pairs for experimental transplantation purposes.
09:42
Analysis of Thermal Expansion via Dilatometry
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2023
Source: J. Jacob Chavez, Ryan T. Davis, and Taylor D. Sparks, Department of Materials Science and Engineering, The University of Utah, Salt Lake City, UT Thermal expansion is extremely important when considering which materials will be used in systems that experience fluctuations in temperature. A high or low thermal expansion in a material may or may not be desirable, depending on the application. For instance, in a common liquid thermometer, a material with a high thermal expansion would be...
06:44
Keratinocyte Colony Formation Assay: An In Vitro Assay to Assess the Ability of Keratinocytes to Grow into Colonies
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2023
In this video, we perform a clonogenic assay of keratinocytes harvested from adult mice to determine the ability of individual cells to proliferate and form colonies. This keratinocyte harvesting technique is useful for studying other downstream aspects of cutaneous chemical carcinogenesis and tumor promotion.
02:38
Isolating Induced Neural Progenitor Cell Colonies Derived From Adult Human Fibroblasts
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2025
This video demonstrates a technique for isolating induced neural progenitor cells (iNPCs) derived from adult human fibroblasts. It outlines the steps involved in isolating the iNPC colonies and culturing them in laminin-coated multi-well plate wells in the presence of suitable culture media that facilitate the continued proliferation and expansion of the iNPC population.
FAQs
Nutrient availability can affect how readily cells reproduce and occupy additional habitat, while other environmental conditions shape survival and outward growth. These influences can change the position or rate of the expanding boundary. Comparing colonies under different conditions therefore helps researchers determine which environmental factors support expansion and which limit population growth across a surface.
Cell signaling can coordinate behaviors among neighboring cells, while extracellular matrix production can alter the shared environment around the colony. Together, these processes may influence how cells remain associated, interact, and extend into available space. Examining them helps explain why expansion reflects collective behavior rather than reproduction and survival operating independently.
An enlarging colony may extend because cells divide, because individuals move across a surface, or because both processes occur together. This distinction matters when interpreting changes in colony size or range: a broader boundary does not by itself identify the relative contribution of reproduction and movement. Researchers can use expansion patterns to investigate these population-level behaviors.
Researchers can track changes in a colony’s size or spatial range over time and compare the resulting expansion patterns under different environmental conditions. Such measurements provide evidence about growth rates and boundary movement without relying only on final population size. The approach is useful for evaluating how nutrients, stress, signaling, or other conditions influence spatial population dynamics.
Changes in the extent or rate of expansion can be used to assess how a colony responds to stress or antimicrobial treatment. Comparing treated and untreated growth patterns may show whether the intervention affects the population’s ability to reproduce, survive, or occupy additional habitat. These measurements connect spatial behavior with treatment response in microbial research.
Spatial expansion provides a way to examine how microorganisms develop within structured environments, including settings where neighboring cells interact and available habitat is limited. The resulting patterns can inform studies of biofilm development, competition, cooperation, and population dynamics. This makes colony expansion relevant not only to laboratory growth studies but also to broader questions in microbial ecology.