Contact Mode

Contact mode is a scanning probe microscopy method in which a nanoscale tip continuously touches a sample surface, enabling high-resolution measurement of biological structures. As the tip raster-scans across the specimen, forces between the tip and surface bend the cantilever; a feedback system adjusts the probe position to maintain a defined interaction force and records the resulting topography. In biology, contact mode can image cell surfaces, membranes, tissues, and immobilized biomolecules, including samples examined in liquid environments. These measurements reveal surface architecture and mechanical interactions, supporting studies of cellular organization, biomolecular structure, and changes caused by treatments or disease.

Contact Mode - Related Videos

Education

JoVE Core - Statistics

What is a Mode?

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2023

The mode is one of the commonly used measures of a central tendency. It is defined as the most frequent value in a data set. There can be more than one mode in a data set if multiple values have the same highest frequency. For instance, suppose that the Statistics exam scores of 20 students are: 50; 53; 59; 59; 63; 63; 72; 72; 72; 72; 72; 76; 78; 81; 83; 84; 84; 84; 90; 93. Here, the mode is 72, as it occurs most frequently, five times. A data set with two modes is called bimodal. For example,...

Research

JoVE Journal - Biology

Contact Mode Atomic Force Microscopy as a Rapid Technique for Morphological Observation and Bacterial Cell Damage Analysis

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Cited by 3 •

2023

Here, we present the application of atomic force microscopy (AFM) as a simple and fast method for bacterial characterization and analyze details such as the bacterial size and shape, bacterial culture biofilms, and the activity of nanoparticles as bactericides.

Characterization of Anisotropic Leaky Mode Modulators for Holovideo

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Cited by 2 •

2016

This work describes fabrication and characterization of anisotropic leaky mode modulators for holographic video.

Contact Angle

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2023

When a solid is dipped inside a liquid, the liquid surface becomes curved near the contact. For some solid–liquid interfaces, the liquid is pulled up along the solid, while for others, the liquid surface is convex or depressed near the solid surface. This phenomenon can be explained using the concept of cohesive and adhesive forces. The adhesive force is the molecular force between molecules of different materials, that is, between the molecules of the solid and the liquid. The cohesive force...

Ventilatory Modes

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2025

Mechanical ventilators are life-saving devices that support or replace spontaneous breathing. They deliver breaths to patients through varying methods known as ventilator modes. Understanding these modes is critical for healthcare providers managing patients with respiratory failure. There are three ventilatory modes: full support, partial support, and spontaneous. These are described below. Full Support Modes Full support modes include controlled mechanical ventilation, continuous mandatory...

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