Cyclic Fed-batch
Cyclic Fed-batch - Related Videos
01:23
Fed-Batch Culture
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2026
Fed-batch culture is a widely used bioprocessing strategy combining aspects of batch culture with controlled substrate feeding to optimize cell growth and product formation. In this semi-closed system, nutrients are strategically added during fermentation, while the accumulated products and biomass remain within the bioreactor until the end of the operation. This controlled addition of substrates allows for better management of growth kinetics, nutrient limitation, and metabolite...
09:40
Batch and Continuous Bioreactors
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2023
Bioreactors are used to grow organisms in large volumes, thereby enabling the production of mass quantities of the target product. These reactors can be batch reactors, which contain all of the components needed for cell growth, or continuous reactors, which have inlet and outlet ports allowing for the addition of fresh growth media and the removal of cell waste. This video presents batch and continuous reactors and demonstrates the use of bioreactors to grow bacteria in the laboratory.
08:37
Cyclic Voltammetry (CV)
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2023
Source: Laboratory of Dr. Kayla Green — Texas Christian University A Cyclic Voltammetry (CV) experiment involves the scan of a range of potential voltages while measuring current. In the CV experiment, the potential of an immersed, stationary electrode is scanned from a predetermined starting potential to a final value (called the switching potential) and then the reverse scan is obtained. This gives a 'cyclic' sweep of potentials and the current vs. potential curve derived from the data is...
08:07
Batch Immunostaining for Large-Scale Protein Detection in the Whole Monkey Brain
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Cited by 5 •
2009
Large-scale immunodetection of target proteins across the entire primate brain is possible by employing novel tissue embedding and sectioning methods combined with the use of creative apparatus for batch staining of multiple free-floating sections at a given time.
01:14
Batch vs Continuous Culture
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2026
Fermentation is a foundational biotechnological process used to produce pharmaceuticals, biofuels, enzymes, and food additives. Among industrial strategies, batch and continuous fermentation are the two most widely applied. Although both rely on microbial conversion of substrates into desired products, they differ markedly in operation, productivity, and suitability for specific applications.Batch fermentation occurs in a closed system in which nutrient media and inoculum are added at the...
FAQs
The process coordinates two opposing actions within each cycle: a concentrated feed replenishes nutrients that may limit growth, while part of the culture volume is withdrawn or harvested. This pairing prevents the cultivation system from simply accumulating volume as cells continue growing. It also helps preserve suitable cell density and environmental conditions for continued biological production.
Concentrated feeds restore limiting nutrients without requiring an equivalent increase in culture volume. This supports ongoing cell or microorganism growth while helping maintain a practical working volume. By supplying nutrients in a concentrated form, the system can continue productive cultivation across repeated cycles and make more efficient use of the available culture space and resources.
Single-batch processing does not use the repeated combination of nutrient replenishment and partial culture removal described for this strategy. Cyclic Fed-batch can extend productive cultivation and enable repeated product collection, which may reduce downtime between production activities. The distinction is especially relevant when maintaining cells or microorganisms over multiple productive periods is valuable.
Repeated cycles can sustain biological activity beyond a single cultivation period while allowing selected culture material or product to be collected more than once. This extended operation may improve productivity and resource use compared with ending the process after one batch. Its value depends on preserving suitable volume, cell density, and environmental conditions throughout the cycles.
A cycle begins with continued cultivation of cells or microorganisms, followed by addition of a concentrated feed to replenish limiting nutrients. A selected portion of the culture is then withdrawn or harvested, and the remaining system continues under suitable volume, density, and environmental conditions. Repeating this sequence supports ongoing growth and additional collection opportunities.
Three conditions identified as central are culture volume, cell density, and the surrounding environmental conditions required for continued growth. Feeding and partial withdrawal must work together so these factors remain suitable rather than drifting as cultivation proceeds. Maintaining this balance allows the biological system to continue producing material across successive cycles instead of ending after one collection.
Researchers may select this strategy when they need sustained cultivation, repeated product collection, or practical resource use at production scale. The overview identifies applications including recombinant proteins, vaccines, metabolites, and other biologically derived products. It can also support studies of cell metabolism, linking process operation with investigation of how cells use nutrients during cultivation.