Dmpc Chapso
Dmpc Chapso - Related Videos
13:20
Online Repetitive Transcranial Magnetic Stimulation of Dorsomedial and Dorsolateral Prefrontal Cortex in Cognition Decision Making, and Cognitive Dissonance
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2025
This protocol presents a more precise and effective method of repetitive transcranial magnetic stimulation of the dorsolateral and dorsomedial prefrontal cortices during an experimental task using neuronavigation and robotic systems. Based on the results, crucial implications are suggested for future online stimulation research.
07:26
High-throughput Crystallization of Membrane Proteins Using the Lipidic Bicelle Method
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Cited by 27 •
2012
Bicelles are lipid/amphiphile mixtures that maintain membrane proteins (MPs) within a lipid bilayer but have unique phase behavior that facilitates high-throughput screening by crystallization robots. This technique has successfully produced a number of high-resolution structures from both prokaryotic and eukaryotic sources. This video describes protocols for generating the lipidic bicelle mixture, incorporating MPs into the bicelle mixture, setting up crystallizations trials (manually as well...
04:46
Synthesis of Nanodisc-Stabilized Membrane Protein Antigens Using a Cell-Free System
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2025
Source: Gilmore, S. F., et al. Cell-Free Scaled Production and Adjuvant Addition to a Recombinant Major Outer Membrane Protein from Chlamydia muridarum for Vaccine Development. J. Vis. Exp. (2022)This video demonstrates the cell-free synthesis of nanodisc-stabilized membrane protein antigens using a dual-compartment system. The device maintains optimal conditions for efficient protein synthesis, proper folding, and stable integration into nanodiscs.
10:09
Thermodynamics of Membrane Protein Folding Measured by Fluorescence Spectroscopy
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Cited by 2 •
2011
This video article details the experimental procedure for obtaining the Gibbs free energy of membrane protein folding by tryptophan fluorescence.
11:57
Saccharomyces cerevisiae Models of Alzheimer's Disease to Screen Genes, Mutations, and Chemicals Affecting Amyloid Beta Production by γ-Secretase
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2025
Here, we describe a yeast system reconstituting human γ-secretase. This system allows for the identification and study of mutations affecting activity and for screening of γ-secretase inhibitors (GSIs). Utilizing the loss of function properties of familial Alzheimer's disease mutants, it is possible to screen for γ-secretase modulators (GSMs).
FAQs
CHAPSO disperses DMPC molecules in aqueous solution, preventing the lipid from behaving only as a bulk, poorly accessible assembly. Its detergent action supports formation of mixed micelles or bicelle-like structures, creating organized environments in which membrane-associated molecules can be examined under experimentally manageable conditions.
The relative amounts of DMPC and CHAPSO, together with temperature, influence how the lipid and detergent organize in water. Under different conditions, the system can favor mixed micelles or bicelle-like assemblies. This responsiveness gives researchers a way to adjust membrane-like organization while maintaining control over the experimental environment.
DMPC-CHAPSO combines membrane-like organization with greater experimental accessibility than a fully complex biological membrane environment. The assemblies can therefore support investigations of membrane protein structure, lipid-protein interactions, and molecular dynamics. This balance helps researchers examine membrane-related behavior while controlling the surrounding model system.
A basic workflow combines DMPC and CHAPSO in an aqueous solution, then selects composition and temperature to obtain the desired mixed micelle or bicelle-like organization. Researchers can introduce the membrane-associated molecule of interest into this controlled environment and then apply an appropriate biophysical measurement to examine its behavior.
Nuclear magnetic resonance spectroscopy is a key biophysical method associated with this system. It can be used to investigate membrane protein structure, lipid-protein interactions, and molecular dynamics within the organized lipid-detergent environment. The model's accessible aqueous setting helps make these membrane-related questions suitable for spectroscopic study.
In biology, the system provides a simplified setting for asking how membrane proteins interact with lipids and how membrane-like environments influence molecular structure and motion. It is useful when researchers need controlled conditions rather than the full complexity of a cellular membrane, while still preserving relevant membrane-associated organization.