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High-throughput Crystallization of Membrane Proteins Using the Lipidic Bicelle Method
 

High-throughput Crystallization of Membrane Proteins Using the Lipidic Bicelle Method

Article doi: 10.3791/3383
January 9th, 2012

Summary January 9th, 2012

Bicelles are lipid/amphiphile mixtures that maintain membrane proteins (MPs) within a lipid bilayer but have unique phase behavior that facilitates high-throughput screening by crystallization robots. This technique has successfully produced a number of high-resolution structures from both prokaryotic and eukaryotic sources. This video describes protocols for generating the lipidic bicelle mixture, incorporating MPs into the bicelle mixture, setting up crystallizations trials (manually as well as robotically) and harvesting crystals from the medium.

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