Dna Crosslinking
Dna Crosslinking - Related Videos
09:06
Preparation of DNA-crosslinked Polyacrylamide Hydrogels
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Cited by 5 •
2014
Our laboratory has developed DNA-crosslinked polyacrylamide hydrogels, a dynamic hydrogel system, to better understand the effects of modulating tissue stiffness on cell function. Here, we provide schematics, descriptions, and protocols to prepare these hydrogels.
03:47
Crosslinking Neuronal Surface Proteins in the Mouse Brain using a Chemical Crosslinking Assay
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2025
This video demonstrates the crosslinking of neuronal surface proteins using Bis-sulfosuccinimidyl suberate or BS3. Isolated and chilled mouse brain is sectioned coronally. The desired region is extracted from the sections, minced, and treated with BS3. BS3 crosslinks surface proteins like GABA receptors, leaving internal proteins unaltered. The reaction is quenched with glycine before processing the samples for further analysis.
10:12
Quantitative Detection of DNA-Protein Crosslinks and Their Post-Translational Modifications
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Cited by 3 •
2023
The present protocol highlights a modified method to detect and quantify DNA-protein crosslinks (DPCs) and their post-translational modifications (PTMs), including ubiquitylation, SUMOylation, and ADP-ribosylation induced by topoisomerase inhibitors and by formaldehyde, thereby allowing the study of the formation and repair of DPCs and their PTMs.
11:15
Nanosponge Tunability in Size and Crosslinking Density
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Cited by 1 •
2017
This article describes a process for tuning the size and crosslinking density of covalently crosslinked nanoparticles from linear polyesters containing pendant functionality. By tailoring synthesis parameters (polymer molecular weight, pendant functionality incorporation, and crosslinker equivalents), a desired nanoparticle size and crosslinking density can be achieved for drug delivery applications.
10:05
Genome-wide Mapping of Drug-DNA Interactions in Cells with COSMIC (Crosslinking of Small Molecules to Isolate Chromatin)
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Cited by 5 •
2016
Identifying the direct targets of genome-targeting molecules remains a major challenge. To understand how DNA-binding molecules engage the genome, we developed a method that relies on crosslinking of small molecules to isolate chromatin (COSMIC).
FAQs
Covalent links prevent the affected DNA strands, or DNA and an attached protein, from separating normally. That physical obstruction can stall replication and transcription because both processes require access to DNA templates. The resulting lesion threatens accurate genome duplication and gene expression, making crosslink recognition and coordinated repair central to cellular genome stability.
Repair must first recognize the abnormal covalent connection, then unhook the damaged DNA so normal processing can resume. Restoration of the resulting DNA structure may require several coordinated pathways rather than one isolated activity. This sequence is important because removing the obstruction alone may not fully restore an intact, functional genome.
Crosslinks can arise during endogenous metabolism or after exposure to external chemical compounds and certain cancer treatments. Considering the source helps researchers relate the lesion to natural cellular processes or environmental and therapeutic exposures. In either case, studying the damage can reveal consequences for genome stability, mutagenesis, and cellular responses to toxic conditions.
A typical repair analysis follows how cells recognize the lesion, unhook the crosslink, and restore damaged DNA. Researchers may examine the contributions of nucleotide excision repair, homologous recombination, and specialized translesion synthesis within that coordinated response. This framework helps connect individual repair activities with the broader outcome of recovering functional genetic material.
Some cancer treatments are among the external agents associated with crosslink formation, so crosslink studies help clarify how those treatments damage DNA and how cells respond. Examining recognition and repair pathways can indicate why lesions persist or are processed. This provides biological context for investigating anticancer drug mechanisms without treating repair as a single-step process.
Cells rely on coordinated repair pathways to manage crosslinked DNA, including nucleotide excision repair, homologous recombination, and specialized translesion synthesis. Studying these responses can help place inherited DNA-repair disorders in the context of genome maintenance. The work also links pathway dysfunction with broader outcomes such as mutagenesis, impaired stability, and altered cellular responses to damage.