Surface Micro-extraction
Surface Micro-extraction - Related Videos
07:16
Extraction of the EPP Component from the Surface EMG
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Cited by 2 •
2009
The endplate potential (EPP) component can be extracted from the surface EMG using a digital filter. The extracted EPP shows oscillation with a frequency of about 30 Hz.
10:36
Prescribed 3-D Direct Writing of Suspended Micron/Sub-micron Scale Fiber Structures via a Robotic Dispensing System
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Cited by 6 •
2015
Here, we present a protocol to fabricate freely-suspended, micron/sub-micron scale polymer fibers and “web-like” structures generated via automated direct writing procedure by means of a 3-axis dispensing system.
08:51
Non-invasive 3D-Visualization with Sub-micron Resolution Using Synchrotron-X-ray-tomography
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Cited by 9 •
2008
We used synchrotron X-ray tomography at the European Synchrotron Radiation Facility (ESRF) to non-invasively produce 3D tomographic datasets with a pixel-resolution of 0.7µm. Using volume rendering software, this allows the reconstruction of internal structures in their natural state without the artefacts produced by histological sectioning.
02:59
Extraction of Viral RNA from Surface-Bound Virions Using a Silica Spin Column
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2026
Source: Klaus, J. P. et al. Highly Sensitive Assay for Measurement of Arenavirus-cell Attachment. J. Vis. Exp. (2016)This video demonstrates the extraction of viral RNA from surface-bound virions on epithelial cells using silica spin columns. The method includes chemical lysis, RNA stabilization, and membrane-based purification for downstream quantification of virus-cell attachment.
09:37
Extracting Metrics for Three-dimensional Root Systems: Volume and Surface Analysis from In-soil X-ray Computed Tomography Data
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Cited by 2 •
2016
A methodology for obtaining visual and quantitative root structure information from X-ray computed tomography data acquired in-soil is presented.
FAQs
When the coated probe, sorptive material, or small solvent phase contacts a target surface, chemical compounds partition from that surface into the extracting phase. The extracted material is then desorbed or transferred for instrumental measurement. This sequence concentrates surface-associated molecules before analysis, allowing a small sampled region to provide chemically informative material.
The probe or sorptive material provides the phase that receives compounds from the contacted surface. Its small format supports localized sampling while limiting the amount of sample and solvent required. After contact, the retained analytes can be desorbed or transferred to an instrument, linking the physical sampling step with chemical measurement.
Localized contact lets investigators examine chemical composition at a defined position rather than treating an entire specimen as uniform. In biological analysis, this supports spatially resolved measurements across tissues, cells, biomaterials, or laboratory surfaces. The approach can therefore retain positional information while using limited material from specimens that may be complex or difficult to replace.
A typical workflow begins by bringing a coated probe, sorptive material, or small solvent phase into contact with the selected surface. Compounds move into the extracting phase during contact. The collected material is then desorbed or transferred for instrumental measurement, producing an analytical record of chemical residues present at the sampled location.
In biology, Surface micro-extraction can be applied to tissues, cells, biomaterials, and laboratory surfaces. The collected compounds may include metabolites, lipids, drugs, and other molecular residues. This range makes the technique useful when researchers need chemical information from a particular biological or experimental surface rather than from a large, homogenized sample.
The method is especially useful when specimens are limited, complex, or require localized examination. Its low sample consumption can help preserve biological material, while direct surface sampling supports spatially resolved analysis. Researchers can consequently investigate molecular residues on specific regions of tissues, cells, biomaterials, or laboratory surfaces without requiring extensive sample preparation described in the source.