Egfr
Egfr - Related Videos
08:12
Identification of EGFR and RAS Inhibitors using Caenorhabditis elegans
0 Views •
Cited by 4 •
2020
The genetically tractable nematode Caenorhabditis elegans can be used as a simple and inexpensive model for drug discovery. Described here is a protocol to identify anticancer therapeutics that inhibit the downstream signaling of RAS and EGFR proteins.
09:38
Establishing Dual Resistance to EGFR-TKI and MET-TKI in Lung Adenocarcinoma Cells In Vitro with a 2-step Dose-escalation Procedure
0 Views •
Cited by 4 •
2017
An in vitro method for establishing dual resistance to an EGFR-TKI and a MET-TKI in cancer cells is described. This method is useful for developing treatments for patients with EGFR-mutations, who exhibit disease progression despite EGFR-TKI treatment with MET-amplification. It can also be modified for inhibitors targeting other molecules.
08:52
Profiling Sensitivity to Targeted Therapies in EGFR-Mutant NSCLC Patient-Derived Organoids
0 Views •
Cited by 7 •
2021
This protocol describes a standardized evaluation of drug sensitivities to targeted signaling inhibitors in NSCLC patient-derived organoid models.
15:05
Deciphering the Structural Effects of Activating EGFR Somatic Mutations with Molecular Dynamics Simulation
0 Views •
Cited by 7 •
2020
The objective of this protocol is to use molecular dynamics simulations to examine the dynamic structural changes that occur due to activating mutations of the EGFR kinase protein.
08:15
Antagonistic Effect of Jiawei Shengjiang San on a Rat Model of Diabetic Nephropathy: Related to EGFR/MAPK3/1 Signaling Pathway
0 Views •
Cited by 1 •
2024
Here, we present a protocol describing network pharmacology and molecular docking techniques to explore the mechanism of action of Jiawei Shengjiang San (JWSJS) in treating diabetic nephropathy.
FAQs
Binding initiates a sequence in which receptor dimers activate the intracellular kinase domain. This domain phosphorylates signaling proteins, creating downstream links to the RAS-RAF-MEK-ERK and PI3K-AKT pathways. These pathways connect the receptor’s surface signal to cellular programs controlling growth, survival, division, and differentiation. EGFR activation therefore converts an external cue into coordinated intracellular responses.
The same signaling system that supports tissue development and repair can become harmful when EGFR is mutated, amplified, or excessively activated. These alterations may strengthen or prolong signals associated with growth, survival, and division, shifting regulation away from normal tissue control. In cancer research, identifying such changes helps explain how EGFR-related signaling may contribute to tumor formation.
EGFR does not produce a single downstream response. Its phosphorylation events can engage both the RAS-RAF-MEK-ERK pathway and the PI3K-AKT pathway, providing multiple routes from receptor activation to cellular behavior. Considering both pathways is important because EGFR-related effects can involve several processes, including growth, survival, division, and differentiation, rather than one isolated outcome.
EGFR functions as a biomarker by indicating a biologically relevant feature of a tumor or disease process. Researchers can consider receptor mutations, gene amplification, or excessive activation when examining EGFR-related cancer biology. This information is valuable because it connects molecular abnormalities with the potential relevance of targeted inhibitors or antibody-based treatments in cancer research.
EGFR findings can guide consideration of targeted inhibitors and antibody-based treatments. These approaches focus on a receptor whose abnormal signaling may contribute to tumor formation, rather than treating cancer biology as entirely nonspecific. The connection between EGFR alterations and therapy makes the receptor important both for investigating disease mechanisms and for developing treatment strategies in cancer research.
EGFR has roles in normal tissue development and repair, so its biological importance extends beyond tumor formation. In a broader biology context, studying this receptor helps connect extracellular growth signals with intracellular pathways that regulate cell behavior. Its dual relevance to healthy processes and disease makes EGFR a useful subject for understanding how signaling becomes either restorative or pathological.