Electrical Activation Mapping
Electrical Activation Mapping - Related Videos
05:15
Monitoring Seizure-Induced Neural Electrical Activity in Brain Slices Using Microelectrode Arrays
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2025
This protocol involves using a microelectrode array (MEA) to record electrical activity in a brain slice exposed to a seizure-inducing drug. The procedure includes positioning the brain slice in an MEA recording chamber, applying electrical stimulation to induce artificial seizures, and analyzing the resulting signals.
02:54
Juxtacellular Recording and Staining for Precise Mapping of Neural Activity in an Awake Rat
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2025
Source: Moore, J. D., et al. Juxtacellular Monitoring and Localization of Single Neurons within Sub-cortical Brain Structures of Alert, Head-restrained Rats. J. Vis. Exp. (2015)This video demonstrates the method of juxtacellular recording and staining in a restrained, awake rat. The procedure involves recording signals from neurons and injecting dye to precisely localize the recording site. This technique allows accurate mapping of neural activity to the brain's anatomical structure.
11:03
Motor Maps
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2023
Source: Laboratories of Jonas T. Kaplan and Sarah I. Gimbel—University of Southern California One principle of brain organization is the topographic mapping of information. Especially in sensory and motor cortices, adjacent regions of the brain tend to represent information from adjacent parts of the body, resulting in maps of the body expressed on the surface of the brain. The primary sensory and motor maps in the brain surround a prominent sulcus known as the central sulcus. The cortex...
14:23
Optical Recording of Electrical Activity in Guinea-pig Enteric Networks using Voltage-sensitive Dyes
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Cited by 10 •
2009
This protocol illustrates how voltage-sensitive dyes enable optical recording of electrical activity from intact neural networks such as the plexuses of the guinea-pig enteric nervous system, with an adjustable resolution that ranges from single-cells to multi-ganglionic circuitry.
07:45
Recording Electrical Activity from Identified Neurons in the Intact Brain of Transgenic Fish
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Cited by 4 •
2013
In this video, we will demonstrate how to record electrical activity from identified single neurons in a whole brain preparation, which preserves complex neural circuits. We use transgenic fish in which gonadotropin-releasing hormone (GnRH) neurons are genetically tagged with a fluorescent protein for identification in the intact brain preparation.
FAQs
Both approaches detect changes in membrane potential across excitable tissue. Electrode arrays sample electrical activity at multiple locations, while voltage-sensitive measurements provide a way to follow signal changes spatially. Researchers then assign activation times to those observations, allowing maps to display conduction pathways and areas where propagation is delayed or abnormal.
Activation times provide the temporal reference needed to interpret where electrical activity appears first and how it spreads across tissue. When timing is assigned at multiple locations, the resulting map can distinguish an organized conduction pathway from regions of delayed or abnormal propagation. This makes spatial patterns interpretable as coordinated biological activity rather than isolated measurements.
Electrical Activation Mapping supports condition-to-condition comparisons by showing whether the spatial pattern of activity changes. Researchers can examine maps for altered conduction pathways, newly apparent delays, or other abnormal propagation patterns. This comparison helps connect differences in electrical organization with disease mechanisms or with the effects of an experimental treatment.
A basic workflow begins by recording electrical activity with an electrode array or voltage-sensitive measurement. The detected membrane-potential changes are associated with their locations, and activation times are assigned. Researchers then display those measurements as a map and inspect the resulting conduction pathways, delayed regions, or abnormal propagation patterns.
In biology, applications span cardiac rhythm, neural signaling, and muscle physiology, so the same mapping logic can be used across several types of excitable tissue. In clinical settings, the approach can help identify arrhythmogenic tissue, meaning tissue associated with abnormal rhythm generation, and can support decisions about interventions.
Researchers can compare electrical activation maps before and after a drug or therapy to evaluate whether electrical function has changed. Relevant outcomes include altered conduction pathways, shifts in activation timing, or differences in delayed and abnormal propagation. These measurements connect an intervention with observable changes in tissue-level electrical organization and help evaluate its effects.