Engineered Rna Target
Engineered Rna Target - Related Videos
02:30
Isolation of Small Regulatory RNA–Bound Bacterial Target RNA Using Affinity Purification
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2025
Source: Mercier, N., et. al., MS2-Affinity Purification Coupled with RNA Sequencing in Gram-Positive Bacteria. J. Vis. Exp. (2021)This video demonstrates the isolation of MS2-tagged small regulatory RNA (sRNA) bound to its bacterial target RNA using affinity purification with a maltose-binding protein (MBP)–MS2 coat protein fusion immobilized on amylose resin.
09:36
RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA
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Cited by 59 •
2018
This RNA pull-down method allows identifying the RNA targets of a long non-coding RNA (lncRNA). Based on the hybridization of home-made, designed anti-sense DNA oligonucleotide probes specific to this lncRNA in an appropriately fixed tissue or cell line, it efficiently allows the capture of all RNA targets of the lncRNA.
12:20
Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons
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Cited by 1 •
2014
Ratiometric bimolecular beacons (RBMBs) can be used to image single engineered RNA transcripts in living cells. Here, we describe the preparation and purification of RBMBs, delivery of RBMBs into cells by microporation and fluorescent imaging of single RNA transcripts in real-time.
11:52
Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations
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Cited by 15 •
2016
We describe a targeted RNA sequencing-based method that includes preparation of indexed cDNA libraries, hybridization and capture with custom probes and data analysis to interrogate selected transcripts for gene expression, mutations, and gene fusions. Targeted RNAseq permits cost-effective, rapid evaluation of selected transcripts on a desktop sequencer.
04:10
Helicase Activity Measurement of a Target Protein Using Biotin-Labeled RNA Duplexes
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2025
In this video, we demonstrate the procedure to determine the helicase activity of a target protein to unwind the biotin-labeled dsRNA substrate. The activity of the enzyme was identified by analyzing the electrophoretic mobility shift, followed by a chemiluminescence assay using chemiluminescent enzyme-conjugated streptavidin.
FAQs
Complementary base pairing connects an engineered RNA target with a guide RNA or nucleic acid probe, while RNA-binding protein recognition relies on selective interaction with the target’s sequence or structure. These mechanisms support different readouts, including detection, cleavage, stabilization, or translation. The choice of recognition mode therefore links target design to the biological process researchers want to control or measure.
A sequence-based target is suited to recognition through complementary base pairing, whereas a structural element can provide a feature recognized selectively by an RNA-binding protein. This design choice affects how specificity is achieved and which downstream response can be monitored or regulated. It also allows researchers to investigate RNA structure alongside gene expression or programmable cellular behavior.
Its interaction can be connected to functional outcomes instead of measurement alone. Depending on the selected guide RNA, nucleic acid probe, or RNA-binding protein, recognition may support cleavage, stabilization, or translation. These outcomes let researchers alter the behavior or persistence of an RNA and examine how controlled RNA interactions influence gene regulation and cellular responses.
Researchers first identify the biological process to direct, monitor, or regulate, then select a suitable RNA sequence or structural element. They design it for recognition by a guide RNA, nucleic acid probe, or RNA-binding protein, and examine the resulting detection, cleavage, stabilization, or translation under defined conditions. This workflow connects molecular design with a measurable biological outcome.
The relevant conditions are those that determine whether the intended recognition and response occur reliably. Researchers should specify the recognition partner, whether a guide RNA, nucleic acid probe, or RNA-binding protein, along with the desired outcome such as detection, cleavage, stabilization, or translation. Defining these parameters helps improve specificity and makes measurements or regulatory effects easier to interpret.
In molecular diagnostics, they can provide designed RNA features for detecting a selected biological signal through complementary pairing or recognition by an RNA-binding protein. In RNA interference, they support sequence-directed regulation of RNA-related processes. Their value in both areas comes from programmable recognition, which can connect a chosen target to detection or gene-regulatory outcomes.
Engineered RNA targets provide experimental handles for studying RNA structure, gene expression, and programmable cellular responses. By linking a designed sequence or structural element to detection, cleavage, stabilization, or translation, researchers can observe how a specific RNA interaction changes a process. This makes the approach relevant to synthetic biology as well as mechanistic studies of RNA behavior.