Engineered T Cells
Engineered T Cells - Related Videos
21:08
Engineering Cell-permeable Protein
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Cited by 12 •
2009
Protein transduction enables the direct delivery of biologically active proteins into cells. In contrast to conventional methods such as DNA transfection or viral transduction this non-invasive paradigm allows highly efficient cellular manipulation in a titratable manner circumventing cellular toxicity and the risk of oncogenic transformation by permanent genetic modification.
04:42
Micro-scale Engineering for Cell Biology
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2007
14:43
Synthesis of Keratin-based Nanofiber for Biomedical Engineering
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Cited by 12 •
2016
Electrospun nanofibers have a high surface area to weight ratio, excellent mechanical integrity, and support cell growth and proliferation. These nanofibers have a wide range of biomedical applications. Here we fabricate keratin/ PCL nanofibers, using the electrospinning technique, and characterize the fibers for possible applications in tissue engineering.
10:28
Using Synthetic Biology to Engineer Living Cells That Interface with Programmable Materials
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Cited by 5 •
2017
This paper presents a series of protocols for developing engineered cells and functionalized surfaces that enable synthetically engineered E. coli to control and manipulate programmable material surfaces.
12:50
Procedure for Lung Engineering
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Cited by 43 •
2011
We have developed a decellularized lung extracellular matrix and novel biomimetic bioreactor that can be used to generate functional lung tissue. By seeding cells into the matrix and culturing in the bioreactor, we generate tissue that demonstrates effective gas exchange when transplanted in vivo for short periods of time.
FAQs
The main design distinction lies in the receptor introduced into the cell. A chimeric antigen receptor, or CAR, and a modified T-cell receptor, or TCR, each provide a way to bind a selected antigen and initiate signaling. Comparing these designs helps researchers examine how receptor architecture influences activation, proliferation, and cytotoxic activity.
Antigen selection determines which molecular target the cells can recognize, while receptor design influences how that recognition is converted into cellular signaling. These choices therefore affect whether the cells can respond selectively and promote useful cytotoxic activity. In disease studies, researchers must relate receptor-target matching to the biological features of the cells or tissues being investigated.
Recognition alone does not determine the result. Cell persistence affects how long the modified population remains available to respond, whereas the surrounding immune environment can shape its activity. Considering both factors helps explain why cells with an appropriate receptor may produce different levels of proliferation or cytotoxicity in different disease or tissue settings.
A study generally begins by selecting a molecular target and choosing a receptor strategy, such as a CAR or modified TCR. Researchers then introduce the relevant genetic design, or otherwise modify the cells, and examine antigen recognition together with signaling, proliferation, and cytotoxic activity. This workflow connects molecular design with measurable cellular behavior.
In cancer immunology, researchers design these cells to recognize antigens associated with tumor cells. The intended outcome is selective engagement followed by signaling, proliferation, and cytotoxic activity directed toward the target population. Studies can then examine how antigen choice, receptor design, persistence, and the immune environment influence tumor-directed responses.
The approach also supports investigations of infection, autoimmunity, and tissue biology. In these settings, researchers can use defined receptor-target interactions to study immune recognition and cellular responses within different biological contexts. The resulting systems help connect antigen recognition with proliferation or cytotoxic activity while examining how the surrounding environment modifies those outcomes.