Experimental Pollen Testing
Experimental Pollen Testing - Related Videos
06:34
A Component-resolved Diagnostic Approach for a Study on Grass Pollen Allergens in Chinese Southerners with Allergic Rhinitis and/or Asthma
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Cited by 4 •
2017
This work describes a protocol that uses a component-resolved approach to study sensitization to grass pollen allergens in a cohort of patients from southern China with allergic rhinitis and/or asthma.
08:11
Collection and Identification of Pollen from Honey Bee Colonies
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Cited by 8 •
2021
We describe methods for collecting corbicular pollen from honey bees as well as protocols for color sorting, acetolysis, and microscope slide preparation of pollen for taxonomic identification. In addition, we present pellet color and taxonomic diversity of corbicular pollen collected from five cropping systems using pollen traps.
06:00
Experimental Protocol for Using Drosophila As an Invertebrate Model System for Toxicity Testing in the Laboratory
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Cited by 10 •
2018
In this paper, we provide a detailed protocol for exposing species in the genus Drosophila to pollutants with the goal of studying the impact of exposure on a range of phenotypic outputs at different developmental stages and for more than one generation.
07:17
Pilot Testing
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2023
Source: Laboratories of Gary Lewandowski, Dave Strohmetz, and Natalie Ciarocco—Monmouth University In any experiment researchers have the challenge of creating experiences for participants that are consistent (i.e., reliable) and authentic (i.e., valid). Yet there are many ways to manipulate any one variable. For example, if you want participants to feel sad, you can have them think of their own sad memory, watch a sad video, or read a sad story. Researchers must find the best way to...
07:47
Anxiety Testing
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2023
Anxiety is a commonly observed behavioral disorder that stems from fear. It is described as increased restlessness, or unpleasant feelings of fear over anticipated events. Experimenters often use rodent models to better understand anxiety disorders in humans. They use different paradigms, like exposing rodents to bright spaces or loud sounds, which are known to induce fear. These tests combined with other interventions such as surgery or drug-administration may assist researchers in pinpointing...
FAQs
Viability stains indicate which pollen grains meet the assay’s criteria for viability, allowing researchers to estimate the quality of a pollen sample without relying only on visible grain structure. This information is useful when comparing plant lines or examining whether storage conditions have preserved pollen suitable for further reproductive study.
On a nutrient medium, pollen is observed under controlled conditions as it hydrates, germinates, and produces a pollen tube. These sequential responses provide functional evidence beyond a viability result alone. Researchers can therefore assess whether pollen performs the early processes needed to contribute to plant fertilization and compare performance among samples.
Viability and germination describe different aspects of pollen performance. A viability stain addresses whether grains satisfy a staining-based viability assessment, whereas culture on nutrient medium reveals hydration, germination, and pollen tube growth. Considering both results gives a broader picture of reproductive function and helps distinguish preserved viability from demonstrated functional activity.
The test conditions should match the question being investigated because pollen responses depend on how hydration, germination, and tube growth are assessed. Nutrient-medium assays require controlled conditions so samples can be compared consistently. Maintaining comparable conditions improves interpretation when evaluating plant lines, storage methods, or effects associated with environmental stress.
Researchers can apply the same viability or nutrient-medium assessment to pollen from different plant lines or storage treatments, then compare the resulting evidence of viability, germination, and tube growth. This approach identifies differences in pollen quality and functional performance, supporting decisions about which material is most suitable for reproductive experiments or controlled crosses.
In breeding programs, pollen testing helps evaluate material before controlled crosses, providing evidence about whether samples may contribute effectively to fertilization. In conservation work, the same assessments support evaluation of pollen quality and storage methods. The results also contribute to studies of plant reproduction, pollination biology, fertility, and environmental stress.