Explant Selection
Explant Selection - Related Videos
06:25
Explant Culture for Developmental Studies
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2023
Explant culture is a technique in which living cells or tissues are removed from an embryo for continued development outside of the organism. This ex vivo approach allows researchers to manipulate and observe developing tissues in ways that are not possible in vivo. Once established, explant culture is frequently used to understand the role of genes and signaling molecules in organogenesis. This video will first introduce the basic principles of explant culture and demonstrate a protocol to...
07:45
Explant Culture of Neural Tissue
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2023
The intricate structure of the vertebrate nervous system arises from a complex series of events involving cell differentiation, cell migration, and changes in cell morphology. Studying these processes is essential to our understanding of nervous system function as well as our ability to diagnose and treat disorders that result from abnormal development. However, neural tissues are relatively inaccessible for experimental manipulations, especially in embryonic mammals. As a result, many...
02:21
Dissection and Mounting of Fruit Fly Larval Brain Explants
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2025
This video demonstrates the dissection and mounting of brain explants from Drosophila larvae. The central nervous system (CNS), attached to eye disks, is extracted from the larvae and then mounted on a glass slide in a physiological saline solution.
02:35
Culturing Murine Spiral Ganglion Neuron Explants on Multielectrode Arrays
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2025
This video demonstrates the process for isolating spiral ganglion explants from the inner ear and co-culturing them with the organ of Corti on a multielectrode array. Spiral ganglia are carefully extracted and sectioned into smaller tissue sections or explants. These explants are cultured on the multielectrode array's active area to promote neuronal process outgrowth.
14:32
Primary Human Bronchial Epithelial Cells Grown from Explants
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Cited by 19 •
2010
Here we describe a detailed method for growing primary human bronchial epithelial cells from explants of human bronchial airway tissue including differentiated growth on an air-liquid interface. This method provides an abundant source of primary cells for investigating the role of the airway epithelium in human lung health and disease.
FAQs
These characteristics influence whether cells remain viable and retain the capacity to regenerate in culture. A suitable developmental stage can improve the likelihood of successful cellular responses, while poor physiological condition may reduce establishment or subsequent development. Selection must also account for genotype, because different genetic backgrounds can vary in regenerative capacity.
Genotype matters because it can influence an explant’s regenerative capacity, even when other culture conditions are appropriate. Accounting for genetic background helps explain variation in cell division, callus formation, shoot development, or root formation. It also improves selection of starting material for reproducible culture, transformation, or propagation objectives.
Freedom from contamination is a critical selection criterion because unwanted biological material can compromise culture initiation and interfere with later development. Surface sterilization is applied after selection, but it does not replace careful source choice. Starting with cleaner material makes the establishment stage more reliable and supports the intended regenerative response.
After placement on nutrient medium, appropriate plant growth regulators provide signals that can direct the cultured material toward different developmental responses. Depending on the conditions, they may stimulate cell division, callus formation, shoot development, or root formation. Thus, regulator choice links the biological potential of the explant to the intended culture outcome.
Following selection, the explant is surface-sterilized and transferred to a nutrient medium containing suitable plant growth regulators. These steps reduce the effects of contamination and provide the chemical environment needed for cultured cells to divide or regenerate. The resulting response can include callus, shoots, or roots, depending on the selected conditions.
In plant biotechnology, this choice supports micropropagation, genetic transformation, and production of disease-free plants. In developmental biology, it provides starting material for examining plant development and tissue regeneration. The value of the approach lies in matching the biological properties of the source material with the question or production goal.