Fatty Acid Oxidation
Fatty Acid Oxidation - Related Videos
12:11
Determination of Fatty Acid Oxidation and Lipogenesis in Mouse Primary Hepatocytes
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Cited by 39 •
2015
De novo lipogenesis and β-fatty acid oxidation constitute key metabolic pathways in hepatocyte, pathways that are perturbed in several metabolic disorders, including fatty liver disease. Here we demonstrate isolation of mouse primary hepatocytes and describe quantification of β-fatty acid oxidation and lipogenesis.
12:24
Methods for the Determination of Rates of Glucose and Fatty Acid Oxidation in the Isolated Working Rat Heart
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Cited by 9 •
2016
The following protocol describes the preparation and utilization of buffers for the quantitative measurement of rates of glucose and fatty acid oxidation in the isolated working rat heart. The methods used for sample analysis and data interpretation are also discussed.
06:03
Quantification of Endothelial Fatty Acid Uptake using Fluorescent Fatty Acid Analogs
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2025
This protocol details an in vitro assay for measuring cellular lipid uptake in endothelial cells upon stimulation with BODIPY-C12 and BODIPY-C16 fluorescent analogs of long-chain and very long-chain saturated fatty acids. This method is efficient and adaptable to other cell types, offering a useful approach for studying lipid metabolism.
02:53
Preparation of Fatty Acid Methyl Esters (FAMEs) from Bacterial Biomass
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2025
Source: Ginies, C., et al. Identification of Fatty Acids in Bacillus cereus. J. Vis. Exp. (2016)This video demonstrates the process of preparing fatty acid methyl esters (FAMEs) from bacterial samples by treating the biomass with an alkaline solution, followed by phase separation and solvent evaporation for downstream analysis.
08:41
Identification of Fatty Acids in Bacillus cereus
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Cited by 8 •
2016
We propose a protocol to identify fatty acids without the need to purify them. It combines information on the retention times with the mass spectra of three types of fatty acid derivatives: fatty acid methyl esters (FAMEs), 4,4-dimethyl oxazoline derivatives (DMOX), and 3-pyridylcarbinyl esters (picolinyl).
FAQs
Activation prepares each fatty acid for subsequent processing, while transport delivers it to the mitochondrial matrix, the compartment identified as the site of beta-oxidation. Keeping these stages conceptually distinct helps explain why fatty-acid use depends not only on the lipid substrate itself, but also on access to the mitochondrial pathway.
Each beta-oxidation cycle removes a two-carbon unit from the fatty-acid chain as acetyl-CoA. At the same time, the pathway generates NADH and FADH2. Repetition therefore converts a long-chain lipid substrate into multiple acetyl-CoA units plus electron-carrying products, linking fatty-acid breakdown to the cell’s broader energy-producing network.
NADH and FADH2 are important because they carry products of fatty acid oxidation onward to processes that produce ATP. Acetyl-CoA enters the citric acid cycle, whereas NADH and FADH2 fuel the electron transport chain. This division explains how beta-oxidation connects the chemical breakdown of lipids with usable cellular energy.
Fasting, exercise, and increased energy demand are contexts in which fatty acid oxidation becomes especially relevant because cells draw on stored lipids to support function. Examining the pathway in these conditions helps relate mitochondrial metabolism to changing energy requirements, rather than viewing lipid breakdown as an isolated biochemical event.
Research on this pathway has relevance to metabolic disorders and mitochondrial disease because both areas concern cellular energy metabolism and mitochondrial function. Studying fatty acid activation, mitochondrial processing, and downstream energy production can provide biological context for investigating how altered lipid use may relate to cellular energy requirements.
The pathway connects stored lipids with mitochondrial energy production, making it relevant to exercise physiology and nutrition. Fasting and exercise provide defined biological contexts in which energy demand changes, while nutrition research considers how cells use available or stored energy sources. Together, these applications extend basic metabolism into whole-organism energy questions.