Fecal Qpcr
Fecal Qpcr - Related Videos
09:57
Quantifying Environmental Microorganisms and Viruses Using qPCR
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2023
Source: Laboratories of Dr. Ian Pepper and Dr. Charles Gerba - The University of Arizona Demonstrating Author: Bradley Schmitz Quantitative polymerase chain reaction (qPCR), also known as real-time PCR, is a widely-used molecular technique for enumerating microorganisms in the environment. Prior to this approach, quantifying microorganisms was limited largely to classical culture-based techniques. However, the culturing of microbes from environmental samples can be particularly challenging, and...
09:21
Quantitative Real-Time PCR using the Thermo Scientific Solaris qPCR Assay
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Cited by 3 •
2010
The Solaris qPCR Gene Expression Assays are novel pre-designed qPCR primer/probe combinations designed to simplify the qPCR process without sacrificing the specificity and robustness of the assay.
08:02
Fecal Glucocorticoid Analysis: Non-invasive Adrenal Monitoring in Equids
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Cited by 6 •
2016
Adrenal activity can be assessed in the equine species by analysis of feces for corticosterone metabolites. The method offers a non-invasive option to assess long term patterns in both domestic and free ranging horses. This protocol describes the enzyme linked immunoassay involved and the associated biochemical validation.
10:58
Isolation of Fecal Bacteria from Water Samples by Filtration
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2023
Source: Laboratories of Dr. Ian Pepper and Dr. Charles Gerba - The University of Arizona Demonstrating Author: Luisa Ikner The quality of water destined for use in agricultural, recreational, and domestic settings is of great importance due to the potential for outbreaks of waterborne disease. Microbial agents implicated in such events include parasites, bacteria, and viruses that are shed in high numbers in the feces of infected people and animals. Transmission to new and susceptible hosts may...
01:18
Detection of Human Fecal Metabolites Using a Bacterial Biosensor
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2026
Begin with a bacterial biosensor culture suspended in a medium.These engineered bacteria produce a transcription factor that, upon binding to a specific fecal metabolite, activates a promoter associated with a fluorescent reporter gene.To this culture, add a filtered human fecal sample containing metabolites, such as lactate, that are involved in host-microbiome interactions.Dilute this mixture with a fresh medium to maintain an optimal reaction volume.Incubate without shaking to mimic the...
FAQs
Fluorescence is monitored during the amplification cycles rather than only after the reaction ends. As the selected nucleic-acid target is amplified, the signal provides a basis for estimating how much target was present in the original stool sample. This makes the result more informative than a simple detected-or-not finding when researchers compare microbial abundance or disease-associated changes.
Sequence-specific primers determine which DNA or RNA sequence is amplified during the reaction. Their targeting gives the assay specificity, allowing researchers to focus on an enteric pathogen, parasite, or microbial marker rather than measuring nucleic acids from the entire stool community. This selectivity connects the measured signal to a defined biological question.
Selecting a defined DNA or RNA target focuses the assay on a particular microorganism, parasite, pathogen, or microbial marker. That focus links the amplification signal to a biological question instead of treating all nucleic acids in stool as equivalent. Target selection therefore supports specific comparisons involving enteric infection, gut microbiota, or disease-associated changes.
A presence-or-absence result indicates whether a selected target was detected, whereas fecal qPCR can also estimate its abundance. This quantitative dimension helps researchers examine differences in microbial levels among samples or conditions. It is especially relevant when studying changes in gut microbiota, infection status, or biological patterns associated with disease.
Researchers can select targets associated with enteric pathogens, parasites, or broader microbial markers. The same targeted strategy also supports investigations of gut microbiota and disease-associated biological changes. By matching the primer-defined target to the research question, investigators can examine either a specific infectious agent or a marker representing a wider intestinal condition.
In clinical investigation, the method can provide sensitive, specific, and quantitative information about targets linked to intestinal infection or disease-associated changes. In epidemiology, measurements of enteric pathogens, parasites, or microbial markers can support comparisons across samples or populations. Its relatively rapid molecular readout makes it useful for investigating patterns of infection and intestinal biology.
Fecal qPCR gives experimental researchers a way to connect selected molecular targets with gut microbial composition, infection, or disease-associated changes. Results can indicate whether a target is present and estimate its abundance, allowing biological conditions to be compared through quantitative measurements. This supports studies that examine intestinal microorganisms and their relationship to broader biological processes.