Fib Lamella
Fib Lamella - Related Videos
09:16
FIBS-enabled Noninvasive Metabolic Profiling
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2014
A description of how to calibrate Förster Resonance Energy Transfer integrated biological sensors (FIBS) for in situ metabolic profiling is presented. The FIBS can be used to measure intracellular levels of metabolites noninvasively aiding in the development of metabolic models and high throughput screening of bioprocess conditions.
08:29
Workflow Using a Cryogenic Coincident Fluorescence, Electron, and Ion Beam Microscope for Targeted Milling of Cells
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Cited by 2 •
2025
This workflow enables lamella production targeting fluorescently labeled biological structures that are small (<1 μm in axial extent) and rare (1 copy per cell) using a cryogenic tri-coincident imaging platform. This platform integrates fluorescence microscopy, focused ion beam milling, and scanning electron microscopy at a single focal position and enables simultaneous fluorescence microscopy while milling.
09:06
Preparation and Cryo-FIB micromachining of Saccharomyces cerevisiae for Cryo-Electron Tomography
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Cited by 2 •
2021
We present a protocol for lamella preparation of plunge frozen biological specimens by cryo-focused ion beam micromachining for high-resolution structural studies of macromolecules in situ with cryo-electron tomography. The presented protocol provides guidelines for the preparation of high-quality lamellae with high reproducibility for structural characterization of macromolecules inside the Saccharomyces cerevisiae.
11:17
Spark Plasma Sintering Apparatus Used for the Formation of Strontium Titanate Bicrystals
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Cited by 5 •
2017
A viable technique for the formation of strontium titanate bicrystals at high pressure and fast heating rate via the spark plasma sintering apparatus is developed.
07:00
Preparing Lamellae from Vitreous Biological Samples Using a Dual-Beam Scanning Electron Microscope for Cryo-Electron Tomography
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Cited by 8 •
2021
Using focused ion beam milling to produce vitreous on-grid lamellae from plunge frozen biological samples for cryo-electron tomography.
FAQs
The two-sided milling geometry controls whether a biological region becomes usable for electron imaging. Focused ion beam removal from opposite faces reduces the specimen to a membrane-like thickness, rather than exposing only one surface. This arrangement creates an electron-transparent path through the remaining material, allowing internal cellular organization to be examined at high resolution.
Vitrification and low temperature help preserve cellular structures in a near-native state while material is removed. This matters because the goal is not merely to create a thin section, but to retain biological organization during preparation. The resulting specimen can therefore support observations of structures and interactions as they exist within cells.
Thickness determines whether electrons can pass through the prepared region. Milling continues until the remaining membrane-like section is thin enough for transmission, making internal structures accessible to cryogenic electron tomography. If the region remains too thick, this imaging requirement is not met; achieving the appropriate thickness is therefore central to the method’s usefulness.
Preparation begins with biological material maintained in a vitrified state under cryogenic conditions. A focused ion beam then removes material from both sides of the selected region, leaving a thin membrane-like section. The critical outcome is a section thin enough for electrons to pass through while cellular structures remain preserved for subsequent high-resolution imaging.
Because the section retains cellular context, it can reveal macromolecular complexes, organelles, and molecular interactions directly within cells. That combination is important for linking molecular architecture with ultrastructural organization, rather than examining each level in isolation. The approach is therefore useful when spatial context is essential to interpreting how biological components are arranged and related.
FIB lamellae support studies in infection, cell biology, and structural biology. In each area, the preparation provides access to high-resolution views of cellular material while retaining near-native organization. This allows researchers to connect visible ultrastructural features with molecular-scale architecture and interactions, giving biological observations both spatial context and structural detail.