Fluorescent Protein Marking
Fluorescent Protein Marking - Related Videos
10:30
Administering and Detecting Protein Marks on Arthropods for Dispersal Research
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Cited by 12 •
2016
Proteins are often used to mark arthropods for dispersal research. Methods for administering internal and external protein marks on arthropods are demonstrated. Protein mark detection techniques, either through indirect or sandwich enzyme-linked immunosorbent assays (ELISAs), are also illustrated.
17:08
In vivo Clonal Tracking of Hematopoietic Stem and Progenitor Cells Marked by Five Fluorescent Proteins using Confocal and Multiphoton Microscopy
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Cited by 11 •
2014
Combinatorial 5 fluorescent proteins marking of hematopoietic stem and progenitor cells allows in vivo clonal tracking via confocal and two-photon microscopy, providing insights into bone marrow hematopoietic architecture during regeneration. This method allows non-invasive fate mapping of spectrally-coded HSPCs-derived cells in intact tissues for extensive periods of time following transplantation.
09:53
Specific Marking of HIV-1 Positive Cells using a Rev-dependent Lentiviral Vector Expressing the Green Fluorescent Protein
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2010
We have developed a lentiviral vector that possesses, in addition to the Tat-responsive LTR, the Rev-response element (RRE) that can regulate reporter gene expression in an HIV-1 Tat- and Rev-dependent fashion. The vector permits the specific detection of replicating HIV in living cells via the expression of GFP.
08:46
Green Fluorescent Protein-based Expression Screening of Membrane Proteins in Escherichia coli
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Cited by 34 •
2015
A streamlined approach to screening for the expression of recombinant membrane proteins in Escherichia coli based on fusion to green fluorescent protein is presented.
08:23
The Bionic Clicker Mark I & II
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Cited by 2 •
2017
A device was created to demonstrate electromyography-based control to a lay audience. After the success of the initial device, a second device was made with greater flexibility in functionality for demonstration and research purposes. This protocol describes the process of building and calibrating both devices.
FAQs
The chromophore is the light-producing part of the fluorescent protein. It forms before the protein can generate its detectable signal. When the chromophore absorbs excitation energy, it emits light at a longer wavelength. That wavelength change gives suitable microscopy a basis for detecting the marked target in cells.
Linking the genes produces a fusion protein that combines the target-associated product with the fluorescent marker. The emitted signal therefore indicates where that fusion protein is located within a cell or biological structure. Tracking this signal helps researchers examine protein localization and cellular dynamics in living systems.
Excitation provides the energy needed for the fluorescent protein's chromophore to emit light, while emission occurs at a longer wavelength. A microscope must therefore be suitable for the relevant light conditions to visualize the marked target. These linked excitation and emission events determine whether the fluorescent label can be observed.
Researchers first select the cell, protein, structure, or gene-expression pattern they want to examine. They then attach a fluorescent protein gene to the relevant target gene so a fusion protein can be produced. After the chromophore forms, suitable microscopy reveals the resulting signal and its location or changes.
This approach is especially useful when researchers need to observe biological activity in living systems rather than analyze only fixed or chemically stained material. Because the marker is genetically encoded, it can support visualization of cell movement, gene expression, protein localization, and other biological dynamics while reducing reliance on endpoint samples.
In biology, the method can connect visible signals with processes such as development, cell signaling, and disease mechanisms. It also supports work in experimental models, where researchers can follow marked cells or proteins and relate their locations or dynamics to broader biological changes. The resulting observations provide spatial and temporal context for these studies.