Fluorescent Screen
Fluorescent Screen - Related Videos
14:53
High-throughput Screening and Biosensing with Fluorescent C. elegans Strains
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Cited by 63 •
2011
A procedure for liquid-based culturing and dispensing of C. elegans strains expressing fluorescent reporter proteins is described that does not require expensive sorting equipment. This approach can be applied to numerous inducible C. elegans genes for drug discovery or biosensing of contaminants.
05:31
A Fluorescent Screening Assay for Identifying Modulators of GIRK Channels
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Cited by 7 •
2012
A real-time screening procedure for identifying drugs that interact with G protein-gated inward rectifier K+ (GIRK) channels is described. The assay utilizes membrane potential-sensitive fluorescent dyes to measure GIRK channel activity. This technique is adaptable for use on a number of cell lines.
02:57
A Fluorescence-Based Assay Using Engineered Lymphocytes for Screening Immunomodulatory Compounds
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2025
This video demonstrates the screening of immunomodulatory compounds via fluorescent-based assay. Transgenic mouse-derived T lymphocytes with green fluorescent protein expression cassettes are treated with these compounds. The enhanced and reduced GFP signals enable the identification of the compounds' stimulatory and inhibitory effects, respectively.
08:46
Green Fluorescent Protein-based Expression Screening of Membrane Proteins in Escherichia coli
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Cited by 34 •
2015
A streamlined approach to screening for the expression of recombinant membrane proteins in Escherichia coli based on fusion to green fluorescent protein is presented.
08:43
A Fluorescence-based Lymphocyte Assay Suitable for High-throughput Screening of Small Molecules
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Cited by 10 •
2017
We present in the current study a novel fluorescence-based assay using lymphocytes derived from a transgenic mouse. This assay is suitable for high-throughput screening (HTS) of small molecules endowed with the capacity of either inhibiting or promoting lymphocyte activation.
FAQs
Excitation light provides the energy needed to stimulate a dye, tagged protein, or reporter, while filters distinguish the resulting emitted light from the illumination used to trigger it. Detectors then capture that separated signal for measurement. This arrangement makes a biological characteristic optically identifiable and helps researchers evaluate cells, molecules, or organisms based on fluorescence.
A fluorescent reporter connects the optical signal to a specific biological target or activity. Dyes, tagged proteins, and other reporters can therefore make different types of traits visible, depending on what they respond to. The selected reporter determines which event becomes measurable, allowing a screen to focus on genetic characteristics, protein location, or another biological feature.
The screen depends on a detectable relationship between fluorescence and the desired characteristic. Reporter responsiveness, excitation of the signal, collection of emitted light, and detector measurement all contribute to whether candidates can be recognized. When these elements produce a usable optical distinction, researchers can measure the signal or select cells, molecules, or organisms for further investigation.
Automated imaging allows fluorescent signals to be collected across large numbers of samples rather than examined only one candidate at a time. Because the method converts biological events into measurable optical information, imaging systems can support systematic analysis and comparison. This scalability is especially useful when researchers need to identify rare or promising candidates from extensive biological sample sets.
A typical workflow links a biological target or activity to a dye, tagged protein, or reporter, then exposes the system to excitation light. Researchers collect the emitted fluorescence with filters or detectors, measure the optical signal, and identify candidates that show the desired characteristic. Selected cells, molecules, or organisms can then undergo further investigation.
This approach is useful when a biological characteristic is difficult to observe directly but can be linked to emitted light. In biology, fluorescent screens support genetic studies, protein localization, cell sorting, and drug discovery. They can reveal where a protein is found, identify cells with a desired trait, or help locate candidates associated with a biological response.
A fluorescent screen can produce measurable signals for comparing samples and can also support selection of candidates with a desired characteristic. Depending on the design, the outcome may be information about genetic traits, protein localization, or a response relevant to drug discovery. Candidates identified through fluorescence can be isolated or examined further in subsequent studies.