Fluorescent Sensor
Fluorescent Sensor - Related Videos
09:57
Imaging Membrane Potential with Two Types of Genetically Encoded Fluorescent Voltage Sensors
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Cited by 5 •
2016
A method for imaging changes in membrane potential using genetically encoded voltage indicators is described.
10:23
Glutamine Flux Imaging Using Genetically Encoded Sensors
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Cited by 7 •
2014
This article will demonstrate how to monitor glutamine dynamics in live cells using FRET. Genetically encoded sensors allow real-time monitoring of biological molecules at a subcellular resolution. Experimental design, technical details of the experimental settings, and considerations for post-experimental analyses will be discussed for genetically encoded glutamine sensors.
09:14
Glovebox and Impurity Sensors
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2023
Source: Tamara M. Powers, Department of Chemistry, Texas A&M University The glovebox provides a straightforward means to handle air- and moisture-sensitive solids and liquids. The glovebox is what it sounds like: a box with gloves attached to one or more sides, which allows the user to perform manipulations within the glovebox under an inert atmosphere. For manipulations under inert atmospheres, chemists can choose between Schlenk or high-vacuum techniques and a glovebox. Schlenk and...
05:15
An Aptamer-based Sensor for Unchelated Gadolinium(III)
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2017
The use of polydeoxynucleotide (44-mer aptamer) molecules for sensing unchelated gadolinium(III) ion in an aqueous solution is described. The presence of the ion is detected via an increase in the fluorescence emission of the sensor.
09:30
Bacterial Detection & Identification Using Electrochemical Sensors
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Cited by 6 •
2013
We describe an electrochemical sensor assay method for rapid bacterial detection and identification. The assay involves a sensor array functionalized with DNA oligonucleotide capture probes for ribosomal RNA (rRNA) species-specific sequences. Sandwich hybridization of target rRNA with the capture probe and a horseradish peroxidase-linked DNA oligonucleotide detector probe produces a measurable amperometric current.
FAQs
Target recognition controls when and where a fluorescent sensor produces a signal. The recognition element interacts with a particular analyte or condition, while the fluorophore supplies the measurable optical response. This coupling links a molecular event to a change in fluorescence, helping researchers relate target presence or state to observed cellular behavior.
The optical readout may appear as a change in emission intensity, a change in wavelength, or both, depending on the sensor and the event being monitored. These changes can track altered pH, ion concentration, analyte binding, or enzymatic activity. Matching the signal type to the biological question helps interpret fluorescence as a molecular indicator.
Fluorescent sensors can complement endpoint assays by reporting events during the time they occur rather than only after a sample is collected and analyzed. In microscopy, their signals can be associated with particular locations in living systems, while temporal observation follows changing physiology, signaling, or protein function. This supports analysis of processes that static measurements may miss.
The monitored target depends on the recognition element and the condition being reported. Examples supported in biology include metabolites, signaling molecules, protein function, pH, ion concentration, analyte binding, and enzymatic activity. This range allows researchers to investigate both chemical changes in cells and functional changes associated with cellular regulation or environmental state.
Researchers pair the sensor's fluorescence readout with microscopy to observe molecular events in defined cellular locations. Live-cell imaging then allows those signals to be followed with high spatial and temporal resolution rather than treated only as a final measurement. This approach helps connect changing fluorescence with cellular physiology, signaling, or protein activity.
Fluorescent sensors make molecular changes accessible during studies of physiology, disease mechanisms, and drug responses. By monitoring metabolites, signaling molecules, cellular environments, or protein function, researchers can examine how biological systems change under different conditions. Imaging-based measurements can therefore reveal dynamic responses that conventional endpoint assays may fail to capture.