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Bacterial Detection & Identification Using Electrochemical Sensors

Bacterial Detection & Identification Using Electrochemical Sensors

Article DOI: 10.3791/4282-v 09:30 min April 23rd, 2013
April 23rd, 2013



We describe an electrochemical sensor assay method for rapid bacterial detection and identification. The assay involves a sensor array functionalized with DNA oligonucleotide capture probes for ribosomal RNA (rRNA) species-specific sequences. Sandwich hybridization of target rRNA with the capture probe and a horseradish peroxidase-linked DNA oligonucleotide detector probe produces a measurable amperometric current.


Bacterial Detection Identification Electrochemical Sensors Blood Glucose Measurement Analyte Detection Signal Transduction Redox Enzyme Amperometric Electrode Sensor Specificity Glucose Sensor DNA Oligonucleotides Sandwich Hybridization Ribosomal RNA (rRNA) Capture Probe Detector Probe Horseradish Peroxidase (HRP) Substrate Oxidation Current Flow
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