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JoVE Journal
Bioengineering

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Bacterial Detection & Identification Using Electrochemical Sensors
 

Bacterial Detection & Identification Using Electrochemical Sensors

Article DOI: 10.3791/4282
April 23rd, 2013

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Summary April 23rd, 2013

We describe an electrochemical sensor assay method for rapid bacterial detection and identification. The assay involves a sensor array functionalized with DNA oligonucleotide capture probes for ribosomal RNA (rRNA) species-specific sequences. Sandwich hybridization of target rRNA with the capture probe and a horseradish peroxidase-linked DNA oligonucleotide detector probe produces a measurable amperometric current.

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