Fly Dissection Protocol

Fly Dissection Protocol is a structured method for opening and examining a fly specimen to reveal its external and internal anatomy. The procedure typically stabilizes the specimen, uses careful cuts to expose tissues, and separates structures for observation under magnification while minimizing damage. In biology, this approach links anatomical form with function, supports identification of organs and body regions, and provides practical training in microscopy, specimen handling, and scientific documentation. Dissection protocols also help researchers and students compare morphology across developmental stages, species, or experimental conditions, making them useful for teaching, anatomy studies, and organismal research.

Fly Dissection Protocol - Related Videos

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JoVE EoE - Drosophila melanogaster (fruit fly)

Drosophila Adult Brain Dissection: A Method in Fly Neurobiology

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2023

This video describes how to dissect and isolate the adult Drosophila brain–a procedure necessary for visualizing the organ, which is otherwise obscured by the cuticle, eye, and tracheal tissue. The example protocol shows a detailed demonstration yielding high-quality preparations that can be used for immunostaining and imaging methods in Drosophila neurobiology.

Dissection of the Midgut from an Adult Fly: A Method for Isolating the Digestive Tract

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2023

In this video, we highlight a dissection of the adult Drosophila intestine and collection of midguts suitable for single-cell dissociation and FACS in order to isolate intestinal stem cells.

Dissection and Mounting of Fruit Fly Larval Brain Explants

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2025

This video demonstrates the dissection and mounting of brain explants from Drosophila larvae. The central nervous system (CNS), attached to eye disks, is extracted from the larvae and then mounted on a glass slide in a physiological saline solution.

Malpighian Tubule Dissection from an Adult Fly: Isolating an Osmoregulatory and Excretory Organ

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2023

This article describes the dissection of the anterior Malpighian tubules (MT) from a female Drosophila fly and an example protocol from Rossano et al. (2017) in which the MTs are prepared for live imaging in a perfusion chamber.

Dissection of the Eye-Brain Complex from Fly Pupae: A Method to Isolate Retinal Tissue

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2023

This video describes how to dissect and isolate the eye-brain complex from Drosophila pupae. The featured protocol demonstrates the procedure yielding high-quality tissue, compatible with, for example, immunostaining, as well as, other gene expression experiments.

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