Gfp Reporter
Gfp Reporter - Related Videos
03:38
Measuring Mycobacterium smegmatis Mistranslation Rates Using a GFP/Luciferase Reporter System
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2025
Source: Chen, Y., et al., Measurement of Specific Mycobacterial Mistranslation Rates with Gain-of-function Reporter Systems. J. Vis. Exp. (2019)This video demonstrates a method to quantify mistranslation in Mycobacterium smegmatis using an inducible GFP and secreted mutant luciferase reporter system. It shows how luminescence resulting from mistranslation is measured and compared to GFP fluorescence to evaluate the impact of a test molecule on translational accuracy.
09:22
Rapid Synthesis and Screening of Chemically Activated Transcription Factors with GFP-based Reporters
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Cited by 11 •
2013
This protocol describes an experimental procedure for the rapid construction of artificial transcription factors (ATFs) with cognate GFP reporters and quantification of the ATFs ability to stimulate GFP expression via flow cytometry.
06:19
In Vivo Imaging of Cx3cr1gfp/gfp Reporter Mice with Spectral-domain Optical Coherence Tomography and Scanning Laser Ophthalmoscopy
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Cited by 5 •
2017
This protocol describes how high-resolution imaging techniques such as spectral domain optical coherence tomography and scanning laser ophthalmoscopy can be utilized in small rodents, using an ophthalmic imaging platform system, to obtain information on retinal thickness and microglial cell distribution, respectively.
10:40
Characterization of Adipocyte-Derived Extracellular Vesicle Secretion Using a CD63-GFP Reporter Mouse Model In Vivo and In Vitro
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2025
The protocol uses an adipocyte-specific CD63-GFP reporter mouse line to visualize and quantify the secretion of adipocyte-derived extracellular vesicles (EVs) and demonstrate their uptake by progenitor cells, revealing a paracrine pathway within adipose tissue.
03:50
A Lentiviral Transcriptional Reporter System to Generate a Glioblastoma Stem Cell Differentiation Reporter Line
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2025
This video demonstrates a technique to generate a glioblastoma stem cell reporter line to identify the differentiation of glioblastoma stem cells (GSCs) into astroglia. It outlines the steps involved in seeding GSCs, transducing them with a lentivirus encoding GFP, and determining GFP reporter expression through flow cytometry.
FAQs
The promoter determines when and where the GFP gene is expressed, allowing fluorescence to report the activity of a selected regulatory element in particular cells or biological contexts. This shifts the experiment from simply marking cells to examining control of gene expression. Researchers can therefore use the signal to follow regulatory behavior during biological studies.
A GFP fusion links fluorescence to the target protein itself. As the target moves within a cell, the attached marker can help reveal its localization and support tracking over time. This arrangement differs from promoter-based reporting, which primarily reflects when the reporter gene is expressed. The choice depends on whether the study focuses on protein position or regulation.
After GFP folds, it forms an internal chromophore, the light-absorbing structure responsible for fluorescence. Blue or ultraviolet excitation provides the energy needed for GFP to emit green light. This chemical step connects gene expression or protein fusion to an optical readout, allowing researchers to visualize the reporter with fluorescence-based microscopy rather than relying only on indirect measurements.
Researchers can place the GFP gene under a promoter selected to reflect a regulatory element, or construct GFP as a fusion with a target protein. They then examine the resulting green fluorescence in cells, using its distribution or appearance to study expression, localization, or activity. This design links the reporter format to the biological question being tested.
It is especially useful when researchers need to observe living systems without invasive detection during the study. GFP can support real-time investigation of developmental biology, gene regulation, genetics, cellular responses, and protein behavior. Because the same fluorescent signal can be followed in living systems, the approach helps connect cellular observations with changes occurring during a biological process.
They can use the signal to monitor gene expression, locate proteins within cells, track cells or proteins, and measure biological responses. The meaning of the fluorescence depends on the reporter design: promoter control emphasizes regulation, whereas a protein fusion emphasizes localization and movement. This distinction helps researchers interpret the same visible marker in relation to different biological questions.