Glass Micropipette Fabrication
Glass Micropipette Fabrication - Related Videos
08:50
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The Looking Glass Self
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The concept of the looking-glass self describes how an individual's self-concept is shaped by their perception of how others see them. This psychological theory, first introduced by sociologist Charles Horton Cooley in 1902, posits that self-identity emerges in a social context and is influenced by the judgments—real or imagined—of others.Research suggests that individuals frequently overestimate how positively others perceive them. This is particularly evident in physical self-perception,...
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Measuring the Mechanical Properties of Glass Fiber Reinforcement Polymer Composite Laminates Obtained by Different Fabrication Processes
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10:31
Micropipette Aspiration of Substrate-attached Cells to Estimate Cell Stiffness
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Cited by 30 •
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Here we describe a quick and simple method to measure cell stiffness. The general principle of this approach is to measure membrane deformation in response to well-defined negative pressure applied through a micropipette to the cell surface. This method provides a powerful tool to study biomechanical properties of substrate-attached cells.
07:36
Fabricating Nanogaps by Nanoskiving
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Cited by 3 •
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The fabrication of electrically addressable, high-aspect-ratio (> 1000:1) metal nanowires separated by gaps of single nanometers using either sacrificial layers of aluminum and silver or self-assembled monolayers as templates is described. These nanogap structures are fabricated without a clean room or any photo- or electron-beam lithographic processes by a form of edge lithography known as nanoskiving.
FAQs
During fabrication, the glass capillary is heated until it softens, while controlled tension draws the material into a taper. The balance between heating and pulling determines the resulting tip geometry and size. This relationship lets researchers produce tools suited to different microscale tasks rather than relying on one fixed pipette design.
Tip geometry determines how precisely a micropipette can interact with biological material or handle very small fluid volumes. Because heating and pulling conditions can be adjusted, researchers can create pipettes with customizable dimensions for specific experimental needs. This flexibility supports both precise measurement and targeted manipulation at the cellular scale.
Clean, inert glass surfaces provide a stable interface for work with cells, membranes, and biological fluids. Their material properties help make fabricated pipettes valuable when researchers need controlled contact with delicate biological systems. This is especially relevant for experiments examining cellular function, membrane properties, or responses to microscale manipulation.
A basic workflow begins with a glass capillary, applies heat until the glass softens, and then pulls it under controlled tension to create a tapered tip. Researchers adjust the heating and pulling conditions to obtain the desired geometry and size. The completed pipette can then be selected for manipulation, measurement, or targeted fluid handling.
Fabricated glass micropipettes support several biological applications, including cell microinjection, patch-clamp recording, electrophysiology, and targeted fluid handling. Each use benefits from the ability to tailor the pipette dimensions to the task. As a result, the same fabrication approach can provide tools for introducing material into cells, recording cellular electrical behavior, or managing small fluid volumes.
In electrophysiology, micropipettes support patch-clamp recording and related measurements of cellular electrical behavior. In broader cell studies, their fine tips enable precise manipulation while their clean, inert glass surfaces assist controlled interaction with biological material. These capabilities help researchers investigate cellular function, membrane properties, and biological responses at the microscale.