Glass Pipettes
Glass Pipettes - Related Videos
06:35
Introduction to Serological Pipettes and Pipettors
0 Views •
2023
The serological pipette is frequently used in the laboratory for transferring milliliter volumes of liquid, from less than 1 ml to up to 50 ml. The pipettes can be sterile, plastic, and disposable or sterilizable, glass and reusable. Both kinds of pipettes use a pipet-aid, for the aspiration and dispensation of liquids. Different sizes of pipettes can be used with the same pipet-aid for a variety of experimental assays. For example, serological pipettes are useful for mixing chemical solutions...
01:28
The Looking Glass Self
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2025
The concept of the looking-glass self describes how an individual's self-concept is shaped by their perception of how others see them. This psychological theory, first introduced by sociologist Charles Horton Cooley in 1902, posits that self-identity emerges in a social context and is influenced by the judgments—real or imagined—of others.Research suggests that individuals frequently overestimate how positively others perceive them. This is particularly evident in physical self-perception,...
05:30
Making Patch-pipettes and Sharp Electrodes with a Programmable Puller
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Cited by 22 •
2008
This video shows how to use a programmable puller to make patch pipettes and sharp electrodes for electrophysiology. The same procedure can be used to make a variety of glass tools, including injection needles.
10:54
In Vivo Three-Dimensional Two-Photon Microscopy to Study Conducted Vascular Responses by Local ATP Ejection Using a Glass Micro-Pipette
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Cited by 10 •
2019
We present an optimized local ejection procedure using a glass micro-pipette and a fast two-photon hyperstack imaging method, which allows precise measurement of capillary diameter changes and investigation of its regulation in three dimensions.
05:12
Pressure-polishing Pipettes for Improved Patch-clamp Recording
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Cited by 13 •
2008
This is a guide to modifying the shape of glass micropipettes. Specifically, by using heat and air pressure the taper is widened without increasing the tip opening, leading to lower pipette resistance. This is critical to obtain low noise recordings of small cells but is useful in many applications.
FAQs
Liquid movement depends on a pressure difference between the pipette interior and the surrounding environment. Lower pressure inside the narrow tube draws liquid upward, while restoring pressure allows the operator to release it in a controlled manner. This mechanism supports either approximate transfer or measured delivery, depending on the pipette design and the degree of control required.
Graduated and volumetric glass pipettes serve different measurement needs. Graduations support reading and transferring varying volumes, whereas a volumetric design is intended for a specified level of measurement precision. Pasteur pipettes instead emphasize dropwise delivery rather than precise volume measurement. Choosing among them depends on whether the task prioritizes volume accuracy, flexibility, or controlled addition.
Glass contributes more than structural rigidity. Its heat resistance allows these pipettes to tolerate sterilization, while its compatibility with laboratory liquids helps reduce chemical interaction with the vessel. These properties matter when researchers handle reagents or biological material while preserving the intended sample environment and, in suitable workflows, preparing the instrument for repeated use.
A practical workflow begins by matching the pipette design to the required volume control, whether measured, approximate, or dropwise. The operator then creates a pressure difference, draws liquid into the tube, and releases it through controlled dispensing. After use, glass's heat resistance and sterilization compatibility can support preparation for repeated use within the laboratory workflow.
In biology, glass pipettes can support the transfer of reagents, buffers, cultures, and biological samples. Their usefulness comes from combining controlled liquid movement with a vessel that can minimize contamination and chemical interaction. This makes them relevant across sample-handling steps in teaching laboratories and research workflows, where reliable movement of small liquid portions is needed.
Their heat resistance and compatibility with sterilization allow glass pipettes to fit workflows that require instrument preparation between uses. Reusability can be particularly useful in teaching laboratories and research settings, provided the laboratory process includes appropriate sterilization. The same material properties also support handling liquids while limiting chemical interaction with the pipette vessel.