Murine Bone Marrow Isolation
Murine Bone Marrow Isolation - Related Videos
07:17
Murine Hind Limb Long Bone Dissection and Bone Marrow Isolation
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Cited by 293 •
2016
Here we present a protocol for the dissection of hind limb long bones (femurs and tibiae) from the laboratory mouse. We further describe a rapid technique for bone marrow isolation from these bones that utilizes centrifugation for removal of bone marrow from the bone marrow space.
09:21
Isolation and Intravenous Injection of Murine Bone Marrow Derived Monocytes
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Cited by 31 •
2014
Here we present a protocol that generates large amounts of murine monocytes from heterogeneous bone marrow for translational applications. In comparison to others, this new method helps reduce the number of sacrificed animals and lowers costs by avoiding expensive methods such as high gradient magnetic cell separation (MACS).
08:41
Femur Window Chamber Model for In Vivo Cell Tracking in the Murine Bone Marrow
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Cited by 8 •
2016
The protocol describes a novel murine femur window chamber model that can be used to track movement of cells in the femoral bone marrow in vivo. Intravital multiphoton fluorescence microscopy is used to image three components of the femoral bone marrow (vasculature, collagen matrix, and neutrophils) over time.
04:00
Isolation and Culture of Bone Marrow Stromal Cells from a Mouse Tibia
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2025
In this video, we demonstrate the isolation and culture of bone marrow stromal cells from the long bones of mice. The isolated adherent BMSCs can be used for further analysis.
08:15
The Preparation of Primary Hematopoietic Cell Cultures From Murine Bone Marrow for Electroporation
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Cited by 13 •
2009
This procedure describes how to establish primary hematopoietic cell cultures from murine bone marrow and is followed by transfection using the Gene Pulser MXCell electroporation system.
FAQs
Flushing applies a suitable buffer to the marrow cavity of an extracted long bone, carrying hematopoietic and stromal cells into a collection vessel. This step converts material contained within the bone into a cell suspension that can be processed further. Effective recovery depends on consistent handling, which helps preserve viability and supports reproducible downstream analyses.
Filtering removes bone fragments and connective tissue from the flushed suspension, producing a cleaner cell preparation. Red blood cell lysis serves a different purpose: it can reduce the red blood cell content when required by a planned downstream analysis. Because lysis is optional, researchers should use it only when the intended experiment requires that additional processing.
Consistent handling helps maintain cell viability and makes preparations more comparable across experiments. Differences in how bones are removed, marrow is flushed, or suspensions are processed can influence the recovered cell material. Standardizing these steps is especially relevant when samples will be compared in flow cytometry, cell culture, transplantation studies, or disease-model investigations.
The workflow begins by removing the mouse long bones and exposing the marrow cavities for processing. A suitable buffer is then used to flush the marrow into a suspension. The suspension is filtered to remove bone fragments and connective tissue, and red blood cell lysis may follow when needed for the selected downstream analysis.
The core materials are the isolated long bones, a suitable flushing buffer, and a filtering step capable of removing bone fragments and connective tissue. An additional red blood cell lysis step may be included according to experimental requirements. These choices determine how the recovered suspension is prepared for later culture, cytometric analysis, transplantation, or other studies.
Prepared marrow cells can be examined by flow cytometry, expanded or maintained in cell culture, or used in transplantation studies. The material also supports investigations of hematopoiesis, immunity, bone biology, and disease models. Its value comes from providing access to hematopoietic and stromal cell populations in a form compatible with several biological research workflows.