Hair Cell Culture
Hair Cell Culture - Related Videos
01:22
Hair Cells
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2019
Hair cells are the sensory receptors of the auditory system—they transduce mechanical sound waves into electrical energy that the nervous system can understand. Hair cells are located in the organ of Corti within the cochlea of the inner ear, between the basilar and tectorial membranes. The actual sensory receptors are called inner hair cells. The outer hair cells serve other functions, such as sound amplification in the cochlea, and are not discussed in detail here. Hair cells are named after...
07:00
Isolation and Culture of Neural Crest Stem Cells from Human Hair Follicles
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Cited by 19 •
2013
This article presents a robust protocol for isolation and culture of neural crest stem cells from human hair follicles.
06:07
Isolation and Culture of Primary Cochlear Hair Cells from Neonatal Mice
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Cited by 1 •
2023
Herein, we present a detailed protocol for isolating and culturing primary cochlear hair cells from mice. Initially, the organ of Corti was dissected from neonatal (aged 3-5 days) murine cochleae under a microscope. Subsequently, cells were enzymatically digested into a single-cell suspension and identified using immunofluorescence after several days in culture.
04:37
Generation of Human Induced Pluripotent Stem Cell-derived Planar Hair-bearing Skin Organoids Using an Air-Liquid Interface Culture System
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Cited by 1 •
2025
This protocol outlines a method for producing skin organoids containing hair follicles from human induced pluripotent stem cells using a planar, physiologically relevant air-liquid interface culture system to replicate skin architecture and function.
06:51
Isolating Hair Follicle Stem Cells and Epidermal Keratinocytes from Dorsal Mouse Skin
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Cited by 23 •
2016
An ideal model for studying adult stem cell biology is the mouse hair follicle. Here we present a protocol for isolating different populations of hair follicles stem cells and epidermal keratinocytes, employing enzymatic digestion of mouse dorsal skin followed by FACS analysis.
FAQs
Controlled exposure to drugs, toxins, or growth signals creates defined experimental conditions in which researchers can examine changes in hair-cell physiology, survival, death, or regeneration. Comparing treated cultures with untreated conditions helps separate responses associated with a particular compound or signal from changes related to maintaining the cells, making the system useful for mechanistic studies.
These cultures support investigation of mechanotransduction, the process by which sensory hair cells respond to mechanical stimulation, as well as injury and recovery-related responses. Because auditory and vestibular cells contribute to hearing and balance, culture experiments can connect cellular changes with the sensory functions affected by damage or altered signaling.
Hair cell culture can be established from tissue explants or from dissociated cells, providing different experimental formats. Explant-based preparations retain the source tissue, whereas dissociation produces a cell-based preparation for studying individual sensory cells and precursor-containing material. The choice allows researchers to match the culture format to questions about physiology, cell death, or regeneration.
Researchers obtain sensory tissue from structures such as the cochlea or utricle, then maintain it as an explant or dissociate it into cells. The preparation is placed in nutrient media under controlled laboratory conditions that support cell survival. Investigators can subsequently apply drugs, toxins, or growth signals and assess the resulting cellular responses.
It is useful when researchers need to examine how potentially harmful compounds affect auditory or vestibular sensory cells. By exposing cultures to toxins and monitoring changes in physiology or cell death, investigators can model ototoxic injury in a controlled setting. The same approach can help evaluate protective compounds before pursuing more complex experiments.
Cultures containing hair cells or their precursor-containing tissues provide tractable systems for examining pathways that limit or promote sensory-cell replacement. Growth signals can be tested for their effects on regenerative responses, while cellular outcomes reveal whether cultures show survival, death, or regeneration-related changes. This is especially valuable when experiments in intact animals are impractical.