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Isolation and Culture of Neural Crest Stem Cells from Human Hair Follicles

JoVE Journal
Neuroscience

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JoVE Journal Neuroscience

Isolation and Culture of Neural Crest Stem Cells from Human Hair Follicles

Isolation and Culture of Neural Crest Stem Cells from Human Hair Follicles

DOI:

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07:00 min

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April 06, 2013

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Ruifeng Yang, Xiaowei Xu

¹Department of Pathology and Laboratory Medicine,School of Medicine, University of Pennsylvania

Chapters

00:05Title
01:12Preparation of Tissue Culture Plates and Media
02:08Extract Hair Follicle Cells from Human Scalp
03:37Isolation and Culture of Hair Follicle NCSCs Using Flow Cytometric Cell Sorting
05:44Representative Hair Follicle Stem Cell Culturing Results
06:42Conclusion

Summary

Automatic Translation

This article presents a robust protocol for isolation and culture of neural crest stem cells from human hair follicles.

Tags

Neural Crest Stem Cells Human Hair Follicles Stem Cell Proliferation Hair Cycle Hair Bulge Outer Root Sheath Epithelial Stem Cells Melanocyte Stem Cells Neural Crest Like Stem Cells Label-retaining Cells Self-renewal Asymmetric Cell Division Gene Expression Pattern Myogenic Cell Lineage Melanocytic Cell Lineage Neuronal Cell Lineage Mesenchymal Lineages Differentiation Potential Mouse Brain

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