Heme Concentration
Heme Concentration - Related Videos
09:43
Measurement of Heme Synthesis Levels in Mammalian Cells
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Cited by 12 •
2015
Altered intracellular heme levels are associated with common diseases such as cancer. Thus, there is a need to measure heme biosynthesis levels in diverse cells. The goal of this protocol is to provide a fast and sensitive method to measure and compare the levels of heme synthesis in different cells.
03:03
Bathophenanthroline Sulfonate-Based Colorimetric Assay: A Simple and Rapid Method for Quantitation of Non-Heme Iron in Mouse Liver Tissue
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2025
In this video, we describe a colorimetric-based method to determine non-heme iron content in mouse liver tissue. Bathophenanthroline sulfonate is used as the chromogenic substrate in the presence of a reducing agent, which converts ferric iron to chelatable ferrous iron.
06:39
Bioluminescence Imaging of Heme Oxygenase-1 Upregulation in the Gua Sha Procedure
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Cited by 40 •
2009
Gua Sha, traditional Chinese therapeutic skin scraping, causes subcutaneous microvascular blood extravasation. We report a protocol of bioluminescence imaging of HO-1-luciferase transgenic mice to demonstrate that Gua Sha upregulates heme oxygenase-1 (HO-1) in multiple organs.
02:41
Concentration Cells
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2020
A concentration cell is a type of a voltaic cell constructed by connecting two almost identical half-cells, both based on the same half-reaction and using the same electrode, differing only in the concentration of one redox species. A concentration cell's potential, therefore, is determined only by the concentration difference of the particular redox species. Consider the following voltaic cell: From the given information, the cell potential of this concentration cell can be calculated using...
09:41
Concentration Dependence - Student Protocol
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2020
Source: Smaa Koraym at Johns Hopkins University, MD, USA Concentration DependenceExpand In this experiment, you will combine varying concentrations of aqueous HCl and sodium thiosulfate to make solid sulfur, which rapidly gathers into opaque yellow particles at a certain sulfur concentration. Since the reaction solution starts clear and colorless, you can easily tell when it reaches that concentration. You'll use the same test tube and total volume each time, so it will take the same amount...
FAQs
Heme contains an iron-containing porphyrin group that interacts with light at specific wavelengths. A spectrophotometric assay uses this optical behavior to estimate how much heme is present from the sample’s absorbance. This makes the measurement useful when researchers need a quantitative indicator of heme abundance rather than only a qualitative observation of hemoprotein-related biology.
Heme may be present within hemoproteins such as hemoglobin, so measuring the sample effectively can require releasing the heme from those protein complexes first. This step helps make the heme available for spectrophotometric assessment. In blood-related studies, that distinction is important because the measured signal can reflect heme associated with hemoglobin rather than only freely present heme.
Changes in heme concentration can reflect altered heme synthesis or breakdown, hemoglobin production, and the development of red blood cells during erythropoiesis. Iron availability can also influence the biological context in which heme is produced or maintained. Consequently, a measured difference may provide clues about blood physiology, cellular metabolism, or disorders involving heme regulation.
A typical workflow begins with a biological sample, followed by treatment that releases heme from hemoproteins when necessary. The prepared sample is then analyzed by measuring absorbance at wavelengths associated with heme. The resulting spectrophotometric signal is used to estimate concentration, allowing researchers to compare heme abundance across samples or experimental conditions.
Measurements can help researchers examine how red blood cell formation relates to hemoglobin production and heme availability. Comparing concentration values across biological samples may support studies of erythropoiesis, blood physiology, and conditions involving abnormal heme synthesis or breakdown. The assay therefore connects a measurable chemical property with processes that determine oxygen transport capacity.
Heme concentration analysis can support investigations of cellular metabolism, enzyme function, oxidative stress, and the biological effects of iron availability. Researchers may use the measurements to assess how cells respond when heme-related pathways are altered. These applications extend beyond hemoglobin research because heme also contributes to biological systems whose function changes with cellular or metabolic conditions.