Heparin Coated Pipette
Heparin Coated Pipette - Related Videos
10:41
Using a GFP-tagged TMEM184A Construct for Confirmation of Heparin Receptor Identity
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Cited by 2 •
2017
A construct encoding TMEM184A with a GFP tag at the carboxy-terminus designed for eukaryotic expression, was employed in assays designed to confirm the identification of TMEM184A as a heparin receptor in vascular cells.
02:21
Purification of Viral Integrase Using Heparin Affinity Chromatography
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2026
Source: Lopez Jr., M. et al. Detection and Removal of Nuclease Contamination During Purification of Recombinant Prototype Foamy Virus Integrase. J. Vis. Exp. (2017)This video demonstrates the procedure for purifying viral integrase from bacterial nuclease contamination using heparin Sepharose column chromatography followed by SDS-PAGE analysis to confirm protein purity.
12:27
Polyelectrolyte Complex for Heparin Binding Domain Osteogenic Growth Factor Delivery
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Cited by 2 •
2016
Self-assembled polyelectrolyte complexes (PEC) fabricated from heparin and protamine were deposited on alginate beads to entrap and regulate the release of osteogenic growth factors. This delivery strategy enables a 20-fold reduction of BMP-2 dose in spinal fusion applications. This article illustrates the benefits and fabrication of PECs.
05:30
Making Patch-pipettes and Sharp Electrodes with a Programmable Puller
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Cited by 22 •
2008
This video shows how to use a programmable puller to make patch pipettes and sharp electrodes for electrophysiology. The same procedure can be used to make a variety of glass tools, including injection needles.
06:35
Introduction to Serological Pipettes and Pipettors
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2023
The serological pipette is frequently used in the laboratory for transferring milliliter volumes of liquid, from less than 1 ml to up to 50 ml. The pipettes can be sterile, plastic, and disposable or sterilizable, glass and reusable. Both kinds of pipettes use a pipet-aid, for the aspiration and dispensation of liquids. Different sizes of pipettes can be used with the same pipet-aid for a variety of experimental assays. For example, serological pipettes are useful for mixing chemical solutions...
FAQs
The coating supports heparin’s anticoagulant action at the sample interface. By enhancing antithrombin activity, heparin promotes inhibition of thrombin and factor Xa. These targets limit progression of the coagulation cascade, reducing the tendency of collected blood to form clots during handling. This mechanism helps keep the sample suitable for subsequent analysis.
Maintaining a fluid sample helps limit coagulation-related changes that could complicate handling and downstream analysis. When clot formation is reduced, the collected material remains more suitable for procedures such as plasma preparation and investigations involving blood cells or circulating molecules. The practical benefit is improved consistency in the specimen presented to later analytical steps.
Compatibility with the intended assay determines whether a heparin coated pipette is an appropriate choice for a given experiment. The coating may reduce coagulation, but the sample still must meet the analytical requirements of the planned test. Checking this relationship helps researchers avoid using a handling approach that could compromise interpretation or limit the usefulness of results.
Use begins at sample collection or transfer, where the treated pipette contacts the blood or other biological material. After handling, the specimen can support plasma preparation, hematology, clinical diagnostic work, or research measurements, depending on the planned assay. This workflow focuses on reducing clot-related changes before the sample reaches downstream analysis.
Plasma preparation is one direct application, while hematology and clinical diagnostics represent broader uses. In research, the pipette can support work involving blood cells or circulating molecules. Across these settings, its value comes from controlling coagulation during handling, so the material transferred into the next stage is less affected by clot formation.
In biology, the main outcome is not simply successful transfer but a specimen better preserved for measurement. Reduced coagulation-related changes can support more reliable downstream analyses, whether the target is plasma, blood cells, or circulating molecules. Researchers should therefore treat the coating as part of sample preparation and judge its usefulness against the requirements of each assay.