Hippocampal Formation
Hippocampal Formation - Related Videos
03:59
Formation and Expansion of Neurospheres from a Hippocampal Tissue
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2025
This video demonstrates the process of neurosphere formation and their expansion from hippocampal tissue. Neural stem and progenitor cells from the postnatal mouse dentate gyrus tissue proliferate and form primary neurospheres, which are then reseeded to form secondary neurospheres.
08:20
Organotypic Hippocampal Slice Cultures
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Cited by 53 •
2011
We describe a method to prepare organotypic hippocampal slices that can be easily adapted to other brain regions. Brain slices are laid on porous membranes and culture media is allowed to form an interface. This method preserves the gross architecture of the hippocampus for up to 2 weeks in culture.
05:13
Co-culturing of Ventral Hippocampal and Nucleus Accumbens
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2025
This video demonstrates the process of preparing ventral hippocampal and nucleus accumbens tissues from mouse brains for co-culture. Neurons extend projections to form synaptic connections between the ventral hippocampal and nucleus accumbens tissues. This method allows studying neuronal interactions between these two brain regions, offering insights into cognition, behavior, and neurological disorders.
03:35
Dissociating and Culturing Neurons from Hippocampal Tissue Samples
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2025
This video demonstrates the dissociation and culture of hippocampal neurons from hippocampal tissue samples using enzymatic and mechanical dissociations. The treatment with Ara-C inhibits the growth of non-neuronal cells, facilitating the selective growth of hippocampal neurons.
02:30
Optogenetic Control of Hippocampal Network Dynamics
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2025
This video demonstrates the method to use optogenetics to modulate hippocampal network dynamics by activating genetically modified light-sensitive interneurons in a mouse brain slice, enhancing theta oscillation synchronization and studying neural circuit behavior.
FAQs
These pathways provide linked routes through which incoming information is processed across the dentate gyrus, hippocampus proper, and subiculum. The perforant path, mossy fibers, and Schaffer collaterals connect successive parts of the circuitry, allowing activity to be transformed as it moves through the network. This organization helps researchers examine how complex experiences are converted into stable neural representations.
Long-term potentiation is an activity-dependent increase in synaptic strength. Within hippocampal formation circuits, such changes can reinforce patterns of neural activity produced by experience, helping stabilize representations associated with learning and memory. Studying this process gives biologists a cellular mechanism for linking brief neural events with longer-lasting changes in how information is retained.
These regions form interconnected components rather than isolated structures. Information can be processed across their linked circuitry through pathways that include the perforant path, mossy fibers, and Schaffer collaterals. Examining the regions together is important because memory and spatial representations emerge from coordinated network activity, not simply from the operation of one anatomical area.
Researchers can investigate how activity in this network corresponds to spatial navigation and to representations of an animal’s or person’s surroundings. Comparing neural activity with learned spatial behavior helps connect circuit processing to navigation. This approach also clarifies how experiences involving locations or movement become organized representations that can support later learning and memory.
Research can examine how altered hippocampal formation activity relates to disrupted memory or navigation in these conditions. The same circuitry that supports learning and spatial representations becomes a useful focus for studying disease-associated changes. Such work helps connect abnormalities in interconnected brain regions and synaptic strength with functional outcomes, including impaired memory formation or navigation.
Because this network supports memory formation and spatial navigation, age-related changes can be examined through their effects on those functions. Researchers may compare how neural representations, circuit activity, or activity-dependent synaptic changes relate to performance across ages. This provides biological context for understanding why aging can affect learning, memory, and the ability to navigate.