Image Generation

Image generation is the computational creation of visual content from text prompts, biological data, or existing images, providing a flexible way to represent and communicate scientific information. Generative models learn statistical patterns from training data and can produce images by transforming random noise into structured outputs, often through repeated denoising guided by a prompt or conditioning signal. In biology, these systems can support microscopy-image simulation, visualization of cells and tissues, generation of synthetic training data, and communication of experimental concepts. Their value depends on careful validation, because generated images may contain artifacts or depict biologically implausible structures.

Image Generation - Related Videos

Research

JoVE EoE - Neuroimaging

Second Harmonic Generation Imaging in a Rat Model to Study Tubulin Defects

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2025

Source: Piazza, V., et al., Label-Free Non-Linear Optics for the Study of Tubulin-Dependent Defects in Central Myelin. J. Vis. Exp. (2023)This video demonstrates the procedure for imaging microtubule abnormalities in a rat brain tissue slice using a two-photon excitation microscope. It employs second harmonic generation (SHG) signals to detect tubulin defects and reduced myelin production.

Generating Primary Cultures for Keratinocyte Live Cell Imaging

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2025

This protocol outlines the culture of freshly obtained (passage 0) keratinocytes for use in live cell imaging.

Generating and Analyzing High-Parameter Histology Images with Histoflow Cytometry

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2024

Described here is a method that can be used to image five or more fluorescent parameters by immunofluorescent microscopy. An analysis pipeline for extracting single cells from these images and conducting single-cell analysis through flow cytometry-like gating strategies is outlined, which can identify cell subsets in tissue sections.

Research

JoVE Journal - Bioengineering
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Hybrid µCT-FMT imaging and image analysis

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Cited by 34 •

2015

We describe a protocol for hybrid imaging, combining fluorescence-mediated tomography (FMT) with micro computed tomography (µCT). After fusion and reconstruction, we perform interactive organ segmentation to extract quantitative measurements of the fluorescence distribution.

Imaging-Guided Bioreactor for Generating Bioengineered Airway Tissue

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Cited by 2 •

2022

The protocol describes an imaging-enabled bioreactor that allows the selective removal of the endogenous epithelium from the rat trachea and homogenous distribution of exogenous cells on the lumen surface, followed by long-term in vitro culture of the cell-tissue construct.

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