Immunofluorescence Fish
Immunofluorescence Fish - Related Videos
13:55
Combined Immunofluorescence and DNA FISH on 3D-preserved Interphase Nuclei to Study Changes in 3D Nuclear Organization
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Cited by 35 •
2013
Here we describe a protocol for simultaneous detection of histone modifications by immunofluorescence and DNA sequences by DNA FISH followed by 3D microscopy and analyses (3D immuno-DNA FISH).
07:25
Basic Methods for the Study of Reproductive Ecology of Fish in Aquaria
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Cited by 2 •
2017
A series of basic methods to enable the study of the reproductive ecology of fish kept in aquaria are described. These are useful protocols for collecting fish using SCUBA, transporting live fish, and observing the reproductive behavior of wild-caught fish kept in aquaria.
02:32
Osmoregulation in Fishes
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2019
When cells are placed in a hypotonic (low-salt) fluid, they can swell and burst. Meanwhile, cells in a hypertonic solution—with a higher salt concentration—can shrivel and die. How do fish cells avoid these gruesome fates in hypotonic freshwater or hypertonic seawater environments? Fish employ osmoregulatory strategies to balance bodily levels of water and dissolved ions (i.e., solutes), such as sodium and chloride. Imagine two solutions separated by a membrane that is permeable to water.
04:57
Immunofluorescence Assay to Detect PM2.5-Mediated DNA Damage in Fish Embryos
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2025
This video demonstrates a highly sensitive immunofluorescence-based assay for detecting PM2.5-induced oxidative damage in the hearts of zebrafish embryos by measuring the levels of 8-OHdG and γH2AX, which indicate DNA damage and double-strand DNA breaks, respectively.
05:12
Swimming Performance Assessment in Fishes
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Cited by 56 •
2011
The lives of the majority of fish are predicated on swimming. This protocol describes techniques for capturing a range of swimming modes available to individual and schooling fish, and includes metrics associated with swimming physiology and behaviour.
FAQs
Specificity comes from the interaction between an antibody and its target antigen. By binding that molecular feature, the antibody marks the protein or cellular structure of interest within the fish sample. Fluorescence-tagged detection then converts that binding event into a visible signal, allowing investigators to distinguish where the target occurs rather than relying only on overall tissue appearance.
The microscope uses selected illumination wavelengths to stimulate the fluorescence-tagged detection system. The resulting emitted light reveals the locations where the antibody-associated signal is present. Choosing appropriate illumination conditions therefore supports visualization of target distribution across cells and tissues, helping researchers examine whether molecular patterns differ among fish samples or experimental conditions.
Changes in fluorescent patterns can indicate that a target protein or cellular structure differs in location or distribution across cells, tissues, or experimental conditions. This spatial information adds context to molecular measurements by showing where a change occurs. In fish biology, such comparisons can connect cellular patterns with development, immune responses, tissue organization, or disease processes.
A basic workflow begins with a fish-cell or tissue sample containing the structures under investigation. Antibodies are applied so they can bind the selected target antigens, followed by fluorescence-tagged detection. The sample is then examined by microscopy under selected illumination wavelengths, and the emitted signal is interpreted according to its location and distribution.
Researchers can use this approach when they need to examine specific proteins or cellular structures within fish cells and tissues. Supported applications include studying development, immune responses, tissue organization, pathogen interactions, and disease processes. It is especially useful when the investigation requires molecular information together with spatial context inside a biological sample.
Fluorescence patterns provide a visual basis for comparing where a target occurs and how its distribution changes between experimental treatments or physiological states. Investigators can assess differences across cells or tissues rather than considering samples as uniform. These outcomes help relate altered molecular localization to biological conditions such as development, immunity, or disease.