Influenza A Virus
Influenza A Virus - Related Videos
10:05
High-throughput Detection Method for Influenza Virus
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Cited by 8 •
2012
This method describes the use of Infrared dye based imaging system for detection of H1N1 in bronchioalveolar lavage (BAL) fluid of infected mice at a high sensitivity. This methodology can be performed in a 96- or 384-well plate, requires <10 μl volume of test material and has the potential for concurrent screening of multiple pathogens.
09:31
Fluorescence-based Neuraminidase Inhibition Assay to Assess the Susceptibility of Influenza Viruses to The Neuraminidase Inhibitor Class of Antivirals
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Cited by 56 •
2017
We describe the use of a phenotypic fluorescence-based neuraminidase inhibition assay to assess the susceptibility of influenza A and B viruses to the neuraminidase inhibitor class of antivirals.
11:31
Generation of Recombinant Influenza Virus from Plasmid DNA
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Cited by 129 •
2010
Rescue of influenza A viruses from plasmid DNA is a basic and essential experimental technique that allows influenza researchers to generate recombinant viruses to study multiple aspects in the biology of influenza virus, and to be used as potential vectors or vaccines.
10:44
Influenza A Virus Studies in a Mouse Model of Infection
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Cited by 38 •
2017
Influenza A viruses (IAVs) are important human respiratory pathogens. To understand the pathogenicity of IAVs and to perform preclinical testing of novel vaccine approaches, animal models mimicking human physiology are required. Here, we describe techniques to evaluate IAV pathogenesis, humoral responses and vaccine efficacy using a mouse model of infection.
06:56
Influenza Virus Propagation in Embryonated Chicken Eggs
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Cited by 105 •
2015
Fertile chicken eggs are widely used to produce large amounts of human influenza A virus as they provide a convenient and cost-effective system to prove high yields of virus.
FAQs
Antigenic drift results from frequent mutations in the viral genome. These gradual genetic changes can alter viral characteristics, allowing circulating strains to differ from earlier versions. Monitoring drift is important because it helps researchers follow strain evolution and informs efforts to improve prevention and disease-control strategies against seasonal respiratory disease.
Reassortment can exchange genome segments when different Influenza A virus variants contribute genetic material to a newly formed virus. This process, called antigenic shift, may produce a virus with substantially different characteristics rather than the gradual changes associated with drift. Such changes are significant because they can potentially enable pandemic spread.
Hemagglutinin supports the virus during entry into host cells, while neuraminidase contributes to the release of newly formed virions. Their separate roles connect the beginning and end of the replication cycle. Because these surface proteins directly affect infection and dissemination, they are important subjects in vaccine development and antiviral research.
Genomic surveillance helps researchers track genetic changes among circulating Influenza A virus strains. By following mutations and genome-segment variation, investigators can identify patterns associated with antigenic drift or possible reassortment. This information supports the recognition of emerging strains and strengthens scientific planning for prevention and disease control.
Research examines viral features that influence infection, replication, and spread, including the surface proteins involved in entry and virion release. Findings can guide vaccine development and antiviral research by identifying relevant viral targets and changing strain characteristics. These efforts aim to improve strategies for preventing or controlling seasonal respiratory disease.
Influenza A virus provides a system for studying how a pathogen uses host cells during replication and how viral components influence infection. Examining these interactions connects genome-directed replication with cell entry and virion release. The resulting knowledge helps explain disease processes and supports broader research into mechanisms of prevention and control.