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Fluorescence-based Neuraminidase Inhibition Assay to Assess the Susceptibility of Influenza Viruses to The Neuraminidase Inhibitor Class of Antivirals
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Fluorescence-based Neuraminidase Inhibition Assay to Assess the Susceptibility of Influenza Viruses to The Neuraminidase Inhibitor Class of Antivirals

Fluorescence-based Neuraminidase Inhibition Assay to Assess the Susceptibility of Influenza Viruses to The Neuraminidase Inhibitor Class of Antivirals

DOI:
10.3791/55570-v

•

09:31 min

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April 15, 2017

•

,
1WHO Collaborating Centre for Reference and Research on Influenza,Peter Doherty Institute for Infection and Immunity

Chapters

  • 00:05Title
  • 01:00Determining the Neuraminidase Activity of Viruses
  • 02:29Assessing Virus Seceptibility to NA Inhibitors Using the NA Inhibition Assay
  • 05:18Calculation of IC50 Values
  • 07:01Results: Susceptibility of Influenza Viruses to the Neuraminidase Inhibitor Class of Antivirals
  • 08:55Conclusion

Summary

Automatic Translation

Automatic Translation

We describe the use of a phenotypic fluorescence-based neuraminidase inhibition assay to assess the susceptibility of influenza A and B viruses to the neuraminidase inhibitor class of antivirals.

Tags

Keywords: Fluorescence-based AssayNeuraminidase InhibitionInfluenza Virus SusceptibilityNeuraminidase Inhibitor AntiviralsMUNANAVirus TitrationNA Activity CurvesZanamivirOseltamivir CarboxylatePeramivirLaninamivir

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