Intracellular Transport
Intracellular Transport - Related Videos
02:35
Visualizing Intracellular Transport of Cargo in Cultured Astrocytes
0 Views •
2025
Source: Creighton, B. A., et al. Visualizing and Analyzing Intracellular Transport of Organelles and Other Cargos in Astrocytes. J. Vis. Exp. (2019)This video demonstrates the visualization of intracellular cargo transport in cultured astrocytes using a fluorescent acidotropic probe that selectively labels acidic endolysosomal vesicles. Time-lapse imaging and Z-stack acquisition with a confocal microscope allow tracking of fluorescently labeled cargo throughout the three-dimensional cell volume.
00:07
Visualizing and Analyzing Intracellular Transport of Organelles and Other Cargos in Astrocytes
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Cited by 4 •
2019
Here we describe an in vitro live-imaging method to visualize intracellular transport of organelles and trafficking of plasma membrane proteins in murine astrocytes. This protocol also presents an image analysis methodology to determine cargo transport itineraries and kinetics.
07:35
Immobilization of Caenorhabditis elegans to Analyze Intracellular Transport in Neurons
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Cited by 11 •
2017
Caenorhabditis elegans (C. elegans) is a good model to study axonal and intracellular transport. Here, I describe a protocol for in vivo recording and analysis of axonal and intraflagellar transport in C. elegans.
07:18
Intracellular Refolding Assay
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Cited by 9 •
2012
In this protocol a method to measure intracellular protein refolding after heat shock is described. This method can be used to study foldases like molecular chaperones and their co-factors or compounds able to influence their activity. Firefly luciferase activity is used as reporter to measure chaperone refolding activity.
01:32
Transport and Acclimatization of Rodents for Research
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2026
All animal procedures described here must be conducted in accordance with institutional animal ethics guidelines and approved by IACUC. All procedures must follow the principles of the 3Rs—Replacement, Reduction, and Refinement—and must be performed by trained personnel. Transporting rodents for research requires procedures that minimize stress and maintain animal welfare during movement to minimize the influence on experimental outcomes. Transport cages should provide adequate ventilation,...
FAQs
Motor proteins provide the moving machinery that carries cellular cargo along cytoskeletal tracks. Kinesin and dynein operate on microtubules, whereas myosin moves cargo along actin filaments. This arrangement links specific cargo to defined intracellular routes and supports delivery to locations required for secretion, organelle positioning, recycling, and cellular communication.
Budding separates cargo from one membrane compartment, while fusion incorporates that cargo into the destination compartment. Both events are required to maintain directional exchange among the endoplasmic reticulum, Golgi apparatus, endosomes, and plasma membrane. Their coordination allows cells to combine membrane movement with the controlled transfer of proteins, lipids, and other cargo.
Transport helps establish where cellular components and cargo are located rather than simply moving them throughout the cell. Directed delivery supports cell polarity, while organelle positioning preserves the spatial organization needed for cell function. Because secretion, recycling, and signaling depend on this organization, transport defects can disrupt both cellular structure and communication.
A useful analysis can follow cargo exchange between the endoplasmic reticulum, Golgi apparatus, endosomes, and plasma membrane. Researchers can compare secretory movement, endocytic uptake, and recycling routes, while also examining how organelles are positioned. Together, these pathways reveal how transport connects membrane compartments and coordinates the movement of cellular materials.
Investigating these transport pathways can clarify how cells secrete materials, internalize substances through endocytosis, recycle components, position organelles, and communicate through signaling. These outcomes show that transport is integrated with both cell structure and function. Studying the routes and their coordination therefore provides context for understanding how cells maintain organized activity.
Transport defects can interfere with the organized movement of cargo and the communication between cellular compartments. Such disruption is relevant to neurodegeneration and infection, where altered trafficking may affect cellular maintenance, signaling, or interactions with invading agents. Examining transport therefore connects fundamental cell biology with disease-related questions about how impaired intracellular organization produces harmful outcomes.