Immobilization of <em>Caenorhabditis elegans</em> to Analyze Intracellular Transport in Neurons

Shinsuke Niwa

doi:10.3791/56690

Journal

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Developmental Biology

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Immobilization of Caenorhabditis elegans to Analyze Intracellular Transport in Neurons

Immobilization of <em>Caenorhabditis elegans</em> to Analyze Intracellular Transport in Neurons

JoVE Journal
Developmental Biology

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JoVE Journal Developmental Biology

Immobilization of Caenorhabditis elegans to Analyze Intracellular Transport in Neurons

Immobilization of Caenorhabditis elegans to Analyze Intracellular Transport in Neurons

7,999 Views

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07:35 min

•

October 18, 2017

DOI:

10.3791/56690-v

DOI:

10.3791/56690-v

•

07:35 min

October 18, 2017

•

1 Views

Shinsuke Niwa¹

¹Frontier Research Institute for Interdisciplinary Sciences and Graduate School of Life Sciences,Tohoku University

Transcript

The overall goal of this time lapse recording is to observe the intracellular transport events in Caenorhabditis elegans neurons.This method can help answer key questions in the cell biology field such as axonal transport and interflagellar transport.The main advantage of this technique is that one can observe intracellular transport in C.Elegans without dissection.Start this experiment by growing transgenic C.elegans strain of interest at 20

Summary

Caenorhabditis elegans (C. elegans) is a good model to study axonal and intracellular transport. Here, I describe a protocol for in vivo recording and analysis of axonal and intraflagellar transport in C. elegans.