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Immobilization of Caenorhabditis elegans to Analyze Intracellular Transport in Neurons
Immobilization of <em>Caenorhabditis elegans</em> to Analyze Intracellular Transport in Neurons
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Immobilization of Caenorhabditis elegans to Analyze Intracellular Transport in Neurons

Immobilization of Caenorhabditis elegans to Analyze Intracellular Transport in Neurons

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07:35 min

October 18, 2017

DOI:

07:35 min
October 18, 2017

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The overall goal of this time lapse recording is to observe the intracellular transport events in Caenorhabditis elegans neurons.This method can help answer key questions in the cell biology field such as axonal transport and interflagellar transport.The main advantage of this technique is that one can observe intracellular transport in C.Elegans without dissection.Start this experiment by growing transgenic C.elegans strain of interest at 20

Summary

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Caenorhabditis elegans (C. elegans) is a good model to study axonal and intracellular transport. Here, I describe a protocol for in vivo recording and analysis of axonal and intraflagellar transport in C. elegans.

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