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Biology

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Intracellular Refolding Assay
 

Intracellular Refolding Assay

Article DOI: 10.3791/3540
January 24th, 2012

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Summary January 24th, 2012

In this protocol a method to measure intracellular protein refolding after heat shock is described. This method can be used to study foldases like molecular chaperones and their co-factors or compounds able to influence their activity. Firefly luciferase activity is used as reporter to measure chaperone refolding activity.

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