Laboratory Culture
Laboratory Culture - Related Videos
13:39
Cell Culture Techniques and Practices to Avoid Contamination by Fungi and Bacteria in the Research Cell Culture Laboratory
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Cited by 1 •
2023
This protocol presents essential cell culture techniques and practices to be used in the research cell culture laboratory to avoid contamination by fungi and bacteria. Within the category of bacteria, special emphasis will be placed on preventing mycoplasma...
10:49
Laboratory-Engineered Glioblastoma Organoid Culture and Drug Screening
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2025
Here, we outline a comprehensive protocol for generating and utilizing laboratory-engineered glioblastoma organoids (LEGO) to investigate genotype-phenotype dependencies and screen potential drugs for glioblastoma treatment.
06:19
Decontamination for Laboratory Biosafety
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2023
Robert M. Rioux and Zhifeng Chen, Pennsylvania State University, University Park, PA Decontamination is essential for laboratory biosafety, as the accumulation of microbial contamination in the laboratory can lead to the transmission of disease. The degree of decontamination can be classified as either disinfection or sterilization. Disinfection aims to eliminate all pathogenic microorganisms, with the exception of bacterial spores on lab surfaces or equipment. Sterilization, on the other hand,...
08:00
Measuring Mass in the Laboratory
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2023
The analytical balance is the common piece of equipment in the scientific laboratory. These highly precise instruments can measure down to ten thousandths or even hundred thousandths of a gram. The triple beam balance is a type of scale that uses counterweights on a three rider beams to counterbalance the sample in the pan. On the other hand, modern analytical balances use a complex system of electronic sensors to accurately mass a substance. The analytical balance is so sensitive that it...
07:27
Making Solutions in the Laboratory
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2023
The ability to successfully make solutions is a basic laboratory skill performed in virtually all biological and chemical experiments. A solution is a homogenous mixture of solute dissolved in bulk liquid known as the solvent. Solutions can be described by their solute concentration, a measure of how much solute is present per unit of solution. In this video, a step-by-step procedure for how to make a water-based, or aqueous, solution for biological applications is presented. The video...
FAQs
Growth depends on matching the culture environment to the biological material being maintained. The nutrient medium provides support, while temperature, pH, oxygen availability, and incubation time influence whether growth proceeds and how consistently it can be observed. Controlling these variables reduces environmental variation, allowing investigators to compare growth patterns or cellular behavior across experiments.
Sterility helps limit contamination that could alter the biological material being studied or make observations difficult to interpret. Because cultures are maintained under conditions intended to support growth, unwanted organisms or materials may also affect the outcome if introduced. Maintaining a sterile nutrient medium and controlled environment therefore improves experimental reliability and supports reproducible biological measurements.
A controlled culture system allows researchers to regulate important conditions rather than observing biological material amid changing environmental influences. Using defined nutrient media, temperature, pH, oxygen availability, and incubation periods makes experimental conditions easier to reproduce. This consistency helps investigators measure growth, compare cellular behavior, and examine biological mechanisms with fewer uncontrolled variables.
A typical workflow begins by preparing a sterile nutrient medium and introducing, or inoculating, the biological material of interest. The culture is then maintained under selected temperature, pH, oxygen, and time conditions. Researchers subsequently observe or measure growth and cellular behavior, using the resulting information to investigate mechanisms, identify biological material, or evaluate experimental responses.
Researchers must select a nutrient medium suited to the biological material and establish conditions that support its maintenance. The main controlled variables identified in the source are temperature, pH, oxygen availability, and incubation time. Sterility is also required to limit contamination. These choices determine whether the culture remains viable and produces interpretable observations.
Laboratory culture supports microbial identification, cell-based experiments, and disease modeling in biological research. It also contributes to drug testing and the production of biological materials, extending its usefulness into medicine and biotechnology. Environmental science can likewise use controlled cultures to examine biological materials under defined conditions rather than relying only on natural settings.
Measurements from a culture can reveal growth patterns and changes in cellular behavior under defined conditions. These observations help researchers investigate biological mechanisms and assess how experimental conditions influence living systems. Depending on the study, culture-based results can support microbial identification, disease models, drug testing, or evaluations of biological material production.