Large Particle Cytometry
Large Particle Cytometry - Related Videos
04:34
Measuring Microglial Uptake of Intravitreally-Delivered Fluorescent Particles Using Flow Cytometry
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2025
This video demonstrates flow cytometry for measuring microglial uptake of fluorescent bioparticles after intravitreal delivery in a mouse eye. Flow cytometry detects live cells expressing CD11b and distinguishes phagocytic microglia cells exhibiting green fluorescence from other myeloid cells, including Ly6C-expressing monocytes and Ly6G-expressing neutrophils.
03:37
Subatomic Particles
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2020
Dalton was only partially correct about the particles that make up matter. All matter is composed of atoms, and atoms are composed of three smaller subatomic particles: protons, neutrons, and electrons. These three particles account for the mass and the charge of an atom. The Discovery of the Electron The first clue about the subatomic structure came at the end of the 19th century when J.J. Thomson discovered the electron using a cathode ray tube. This apparatus consisted of a sealed glass...
10:43
Flow Cytometry Purification of Mouse Meiotic Cells
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Cited by 65 •
2011
An efficient method to obtain highly purified viable meiotic fractions from mouse testis is described, which combines a refined cell dissociation protocol with fluorescent activated cell sorting (FACS). This method takes advantage of differences in the DNA content and nuclear density of discrete meiotic fractions.
07:02
Quantifying Human Norovirus Virus-like Particles Binding to Commensal Bacteria Using Flow Cytometry
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Cited by 8 •
2020
The goal of this protocol is to quantify binding of the eukaryotic pathogen human norovirus to bacteria. After performing an initial virus-bacterium attachment assay, flow cytometry is used to detect virally-bound bacteria within the population.
17:07
Flow Cytometry and Fluorescence-Activated Cell Sorting (FACS): Isolation of Splenic B Lymphocytes
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2023
Source: Perchet Thibaut1,2,3, Meunier Sylvain1,2,3, Sophie Novault4, Rachel Golub1,2,3 1 Unit for Lymphopoiesis, Department of Immunology, Pasteur Institute, Paris, France 2 INSERM U1223, Paris, France 3 Université Paris Diderot, Sorbonne Paris Cité, Cellule Pasteur, Paris, France 4 Flow Cytometry Platfrom, Cytometry and Biomarkers UtechS, Center for Translational Science, Pasteur Institute, Paris, France The overall function of the immune system is to defend the body against infectious...
FAQs
The enlarged or specialized flow path allows biological particles with substantial size or structural complexity to move through the system more reliably. This design helps reduce clogging and limits disruption of aggregates, spheroids, organoids, or tissue fragments during measurement. Preserving particle integrity supports more representative analysis of multicellular structure and composition.
Light scattering provides information related to particle size and structure, whereas fluorescence reveals labeled biomarkers or other detectable components. Used together, these signals can distinguish physical characteristics from molecular or compositional features. The combined measurements therefore support a more complete characterization of complex biological particles than either signal would provide alone.
Conventional flow cytometry is not always suitable for particles that are unusually large, heterogeneous, or prone to disrupting the flow path. Large Particle Cytometry adapts the transport system for these challenging samples, reducing clogging and particle damage. This expands cytometric analysis to multicellular systems that may be measured unreliably with standard instruments.
Maintaining particle integrity helps ensure that measured size, structure, composition, and biomarker signals reflect the original biological sample rather than damage caused during transport. This is particularly important for aggregates, spheroids, organoids, and tissue fragments, whose organization contributes to their biological meaning and whose heterogeneity may be lost if particles are disrupted.
Suitable samples include cell aggregates, spheroids, organoids, tissue fragments, and other complex particle populations that are too large or heterogeneous for reliable conventional flow cytometry. The approach is especially relevant when researchers need to examine multicellular systems or culture-derived particles while retaining information about variation in size, structure, composition, or labeled biomarkers.
The sample is transported through an enlarged or specialized flow path, where lasers interrogate the biological particles. Detected light scattering and fluorescence signals are then used to characterize features such as size, structure, composition, and labeled biomarkers. This workflow enables high-throughput measurement while helping limit clogging and disruption during particle passage.
In biology, the method can quantify heterogeneity across complex particle populations, monitor growth, and evaluate responses to treatment. It also supports analysis of multicellular systems and culture aggregates that standard cytometers may not handle reliably. These measurements help researchers compare particle populations and track changes in their physical or labeled biological characteristics.