Large-vessel Blood Collection
Large-vessel Blood Collection - Related Videos
01:30
Blood Collection in Rabbits
0 Views •
2026
All animal procedures described here must be conducted in accordance with institutional animal ethics guidelines and approved by IACUC. All procedures must follow the principles of the 3Rs—Replacement, Reduction, and Refinement—and must be performed by trained personnel. Blood sampling is commonly performed in laboratory rabbits for research and clinical applications. The collection site is selected based on the required sample volume, sampling frequency, and study objectives. As a general...
05:12
Repeated Blood Collection from Tail Vein of Non-Anesthetized Rats with a Vacuum Blood Collection System
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Cited by 25 •
2017
Here, we describe a simple tail vein blood sampling method in non-anesthetized rats using a vacuum extraction tube system. This method reduces the risk of direct exposure to blood and simplifies taking multiple samples from a single venipuncture.
10:01
En Face Preparation of Mouse Blood Vessels
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Cited by 20 •
2017
A procedure for making en face preparations of the mouse carotid artery and aorta is described. Such preparations, when immunofluorescently stained with specific antibodies, enable us to study localization of proteins and identification of cell types within the entire vascular wall by confocal microscopy.
07:03
An in vivo Assay to Test Blood Vessel Permeability
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Cited by 335 •
2013
We are presenting an in vivo assay to test blood vessel permeability. This assay is based on intravenous injection of a dye and subsequent visualization of its diffusion into interstitial spaces.
01:33
Blood Collection in a Pig Model
0 Views •
2026
All animal procedures described here must be conducted in accordance with institutional animal ethics guidelines and approved by IACUC. All procedures must follow the principles of the 3Rs—Replacement, Reduction, and Refinement—and must be performed by trained personnel. Blood collection is a common procedure in swine research and clinical studies. The choice of sampling site depends on the animal's age, body size, the volume of blood required, the study objectives and approved IACUC...
FAQs
Vessel selection influences the amount of blood obtained, the ease of withdrawal, and the likelihood of trauma during sampling. Researchers should choose a major vessel appropriate for the study and use restraint that limits movement without adding unnecessary stress. A suitable choice supports efficient collection while helping reduce tissue injury, contamination, and changes in blood composition.
The additive must match the intended analysis because anticoagulants serve different collection purposes. They can preserve plasma or prevent clot formation, whereas an analysis requiring another blood fraction may require different handling. Selecting the appropriate tube and additive helps maintain the specimen in a usable state and improves the reliability of downstream hematology, chemistry, immunology, or molecular results.
Trauma, contamination, inadequate restraint, and poor handling can change the specimen before analysis. These influences may affect blood composition and make results less representative of the original biological state. Sterile technique, careful vessel entry, appropriate collection containers, and prompt attention to specimen handling collectively help preserve sample quality and limit avoidable experimental variation.
A basic workflow includes selecting an appropriate vessel, preparing sterile equipment, applying suitable restraint, entering the vessel with a needle or catheter, and withdrawing blood into the tube intended for the analysis. The sample then requires careful handling to preserve its required fraction. Coordinating vessel choice, collection equipment, and tube type reduces preventable problems during analysis.
This approach becomes useful when the study requires a relatively high-volume specimen or when a small sample cannot support the planned analyses. The additional volume can accommodate work across several biological measurements, including hematology, clinical chemistry, immunology, or molecular studies. The method is therefore especially relevant when sample quantity limits the scope or reliability of testing.
Large-vessel blood collection can provide material for hematologic measurements, clinical chemistry, immune-related analyses, and molecular studies. Its value comes from supplying enough specimen for the selected testing plan while preserving sample quality through suitable additives and handling. In biology research, careful collection therefore links specimen acquisition to more dependable interpretation of multiple blood-based outcomes.