Liver Dysfunction
Liver Dysfunction - Related Videos
06:26
Mouse Model of Metabolic Dysfunction-Associated Steatotic Liver Disease with Fibrosis
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Cited by 1 •
2025
Mouse model of metabolic dysfunction-associated steatotic liver disease (MASLD) with metabolic dysfunction, hepatic gene expression changes, and liver histopathological alterations that resemble human MASLD, including fibrosis that progresses to advanced fibrosis stage 3. This model can be used in studies of MASLD pathophysiology and in pre-clinical studies of new therapies.
07:03
In Vitro Modeling of Fat Deposition in Metabolic Dysfunction-Associated Steatotic Liver Disease
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Cited by 1 •
2024
This article describes the use of oleic acid-induced HepG2 cells as a model for metabolic dysfunction-associated steatotic liver disease.
12:35
In vivo Liver Endocytosis Followed by Purification of Liver Cells by Liver Perfusion
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Cited by 14 •
2011
The study of liver sinusoidal endothelial cells (SECs) must be performed with primary cells obtained from the animal as no cell lines exist. This method relies on liver digestion and differential centrifugation for SEC purification for subsequent culturing and experimentation.
12:13
Ex Situ Normothermic Machine Perfusion of Donor Livers
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Cited by 21 •
2015
Here we present a protocol describing oxygenated ex situ machine perfusion of donor liver grafts. This article contains a step by step protocol to procure and prepare the liver graft for machine perfusion, prepare the perfusion fluid, prime the perfusion machine and perform oxygenated normothermic machine perfusion of the liver graft.
09:24
Decellularization and Recellularization of Whole Livers
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Cited by 39 •
2011
Perfusion decellularization is a novel technique to produce whole liver scaffolds that retains the organ's extracellular matrix composition and microarchitecture. Herein, the method of preparing whole organ scaffolds using perfusion decellularization and subsequent repopulation with hepatocytes is described. Functional and transplantable liver grafts can be generated using this technique.
FAQs
Hepatocyte injury disrupts several coordinated activities at once. Reduced cellular performance can impair nutrient processing, medication handling, removal of harmful compounds, glucose and lipid regulation, bile production, and synthesis of blood proteins. Because these functions are interconnected, injury in liver cells can create both metabolic disturbances and changes in blood chemistry rather than a single isolated abnormality.
These measures reflect different consequences of impaired liver activity. Liver enzymes support assessment of cellular injury, whereas bilirubin relates to the organ’s handling of a substance associated with bile processing. Tests of synthetic function indicate whether the liver can produce important blood proteins, including proteins involved in clotting. Considering all three areas gives a broader assessment than relying on one measurement.
The overview identifies inflammation, fibrosis, cirrhosis, and organ failure as conditions within the spectrum studied in liver dysfunction. This progression reflects increasing disruption of liver structure and hepatocyte activity. Fibrosis represents a key disease process in that spectrum, while cirrhosis and failure indicate more advanced impairment. Studying these stages helps researchers connect cellular injury with declining organ performance.
Liver regeneration is an important research context because investigators examine how the organ responds after injury and why that recovery may be insufficient in disease. Comparing regenerative responses with persistent dysfunction can clarify disease mechanisms and identify possible therapeutic targets. This work also helps relate hepatocyte activity to broader outcomes, including progression toward fibrosis, cirrhosis, or organ failure.
Assessment commonly combines measurements of liver enzymes, bilirubin, and synthetic function. Enzyme results help indicate injury, bilirubin measurements provide information related to bile handling, and synthetic-function measures show whether blood-protein production is affected. Interpreting these categories together supports evaluation of the type and extent of impairment and provides outcomes that can be compared across disease studies.
Experimental models are used when researchers need to investigate disease mechanisms, evaluate therapeutic targets, or examine liver regeneration in a controlled research setting. They can help connect changes in hepatocyte activity with broader biological outcomes such as inflammation and fibrosis. These models complement measurements from liver assessment by allowing investigators to study processes that are difficult to isolate in an intact organism.
Liver dysfunction links cellular biology to whole-organism regulation because the liver processes nutrients and medications, removes harmful compounds, and helps control glucose and lipid balance. Impairment therefore has consequences beyond the liver itself. In biology and biomedical research, this connection makes liver dysfunction useful for studying how organ injury alters metabolism, chemical handling, blood composition, and potential treatment responses.