Lyophilization Extraction
Lyophilization Extraction - Related Videos
07:27
The Development of Lyophilized Loop-mediated Isothermal Amplification Reagents for the Detection of Coxiella burnetii
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Cited by 7 •
2016
We described here a simple loop-mediated isothermal amplification (LAMP) method using lyophilized reagents for the detection of C. burnetii in patient samples.
11:14
Mass Spectrometric Approaches to Study Protein Structure and Interactions in Lyophilized Powders
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Cited by 19 •
2015
Here, we present detailed protocols for solid-state amide hydrogen/deuterium exchange mass spectrometry (ssHDX-MS) and solid-state photolytic labeling mass spectrometry (ssPL-MS) for proteins in solid powders. The methods provide high-resolution information on protein conformation and interactions in the amorphous solid-state, which may be useful in formulation design.
02:31
Extracellular Matrix-derived Foam Fabrication via Lyophilization: A Procedure to Generate Biological Scaffolds from ECM of Decellularized Tissues
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2023
This video describes the method for synthesizing pure ECM-derived foams without the need for chemical crosslinking. These ECM-derived foams find applications as advanced 3D in vitro cell culture models or as pro-regenerative bioscaffolds.
08:32
Extraction of Tissue Antigens for Functional Assays
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2012
A simple protocol for preparing extracts of human tissue to be used as a source of antigens in functional T-cell assays is described. This method allows T-cell responses to tissue-derived antigens to be measured in vitro.
02:55
Extraction - Concepts
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2020
Extraction Extraction is a common technique used in organic chemistry to isolate a target compound. In the extraction process, a solute is transferred from one phase to another to separate it from unreacted starting materials or impurities. Extraction is also used to facilitate the isolation of a solute from a reaction solvent that is difficult to remove by evaporation, such as a solvent with a high boiling point. Generally, there are three types of extractions. First, in solid-liquid...
FAQs
Reduced pressure enables the dried sample to lose ice by sublimation, rather than requiring the ice to become liquid first. This removes water from the frozen biological material and produces a dry matrix suitable for subsequent rehydration or solvent treatment. The pressure-dependent dehydration step therefore connects physical preservation with chemical recovery.
The porous matrix created after ice removal can be rehydrated or contacted with a solvent, allowing target molecules to leave the dried biological material. Its structure therefore supports the transition from dehydration to extraction. This is especially useful when researchers need to recover metabolites, proteins, or small molecules for biochemical and analytical studies.
Water removal supports storage of biological materials while reducing water that could interfere with later extraction and analysis. Drying also concentrates the sample material into a form that can be rehydrated or treated with solvent when needed. These effects make the approach relevant when preservation and downstream molecular recovery must occur within the same workflow.
A typical workflow begins by freezing the biological sample, followed by placement under reduced pressure so its ice sublimates into vapor. After dehydration, the resulting dry matrix is either rehydrated or treated with a solvent to release compounds of interest. The recovered material can then support downstream biochemical or analytical studies.
Researchers may choose this approach when a biological sample must be preserved during storage and later processed for molecular recovery. Removing water before extraction can limit water-related interference and creates a dry material that can be rehydrated or solvent-treated. It is therefore suited to workflows combining sample preservation, concentration, and downstream analysis.
The method can be applied to tissues, cells, microbial samples, and other biological matrices. Depending on the study, solvent-based recovery may target metabolites, proteins, or small molecules. The resulting extracts can contribute to biochemical and analytical investigations, making the approach relevant across different biological sample types rather than being restricted to one matrix.