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JoVE Journal
Chemistry

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Mass Spectrometric Approaches to Study Protein Structure and Interactions in Lyophilized Powders
 

Mass Spectrometric Approaches to Study Protein Structure and Interactions in Lyophilized Powders

Article doi: 10.3791/52503
April 14th, 2015 Usage Statistics

Summary April 14th, 2015

Here, we present detailed protocols for solid-state amide hydrogen/deuterium exchange mass spectrometry (ssHDX-MS) and solid-state photolytic labeling mass spectrometry (ssPL-MS) for proteins in solid powders. The methods provide high-resolution information on protein conformation and interactions in the amorphous solid-state, which may be useful in formulation design.

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