Microbial Analysis

Microbial analysis is the systematic study and identification of microorganisms, their abundance, characteristics, and activities in biological or environmental samples. It combines methods such as microscopy, culture-based testing, biochemical assays, and molecular techniques to detect microbes, characterize their traits, and measure changes in microbial communities. In biology, these analyses help researchers investigate microbial diversity, metabolism, host–microbe interactions, and responses to environmental conditions. The resulting data support applications ranging from diagnosing infectious diseases and monitoring contamination to studying ecosystems, developing biotechnology, and understanding how microorganisms influence human, animal, and plant health.

Microbial Analysis - Related Videos

Research

JoVE Journal - Environment
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Extraction and Analysis of Microbial Phospholipid Fatty Acids in Soils

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Cited by 133 •

2016

Phospholipid fatty acids provide information about the structure of soil microbial communities. We present methods for extraction from soil samples with a single-phase chloroform mixture, fractionation of extracted lipids using solid phase extraction columns, and methanolysis to produce fatty acid methyl esters, which are analyzed by capillary gas chromatography.

Research

JoVE Journal - Environment
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Microbial DNA Analysis in the Field Using a Biological Extraction Field Kit and a Field qPCR Unit

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2026

Reliable and accurate forms of field-deployable DNA analysis are crucial for many industries. This paper offers two methods for environmental microbial analysis in the field: 1) the isolation of microbial DNA from environmental samples using a biological extraction field kit, and 2) the analysis of DNA via the field qPCR unit.

Research

JoVE Journal - Biology
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Biology of Microbial Communities - Interview

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Cited by 3 •

2007

Research

JoVE Journal - Biology
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Pyrosequencing for Microbial Identification and Characterization

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Cited by 21 •

2013

Pyrosequencing is a versatile technique that facilitates microbial genome sequencing that can be used to identify bacterial species, discriminate bacterial strains, and detect genetic mutations that confer resistance to anti-microbial agents. In this video, the procedure for microbial amplicon generation, amplicon pyrosequencing, and DNA sequence analysis will be demonstrated.

Microfluidic Picoliter Bioreactor for Microbial Single-cell Analysis: Fabrication, System Setup, and Operation

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Cited by 75 •

2013

In this protocol the fabrication, setup and basic operation of a microfluidic picoliter bioreactor (PLBR) for single-cell analysis of prokaryotic microorganisms is introduced. Industrially relevant microorganisms were analyzed as proof of principle allowing insights into growth rate, morphology, and phenotypic heterogeneity over certain time periods, hardly possible with conventional methods.

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