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JoVE Journal
Biology

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Pyrosequencing for Microbial Identification and Characterization
 

Pyrosequencing for Microbial Identification and Characterization

Article DOI: 10.3791/50405
August 22nd, 2013

Chapters

Summary August 22nd, 2013

Pyrosequencing is a versatile technique that facilitates microbial genome sequencing that can be used to identify bacterial species, discriminate bacterial strains, and detect genetic mutations that confer resistance to anti-microbial agents. In this video, the procedure for microbial amplicon generation, amplicon pyrosequencing, and DNA sequence analysis will be demonstrated.

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