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JoVE Journal
Environment

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Extraction and Analysis of Microbial Phospholipid Fatty Acids in Soils
 

Extraction and Analysis of Microbial Phospholipid Fatty Acids in Soils

Article doi: 10.3791/54360
August 26th, 2016

Summary August 26th, 2016

Phospholipid fatty acids provide information about the structure of soil microbial communities. We present methods for extraction from soil samples with a single-phase chloroform mixture, fractionation of extracted lipids using solid phase extraction columns, and methanolysis to produce fatty acid methyl esters, which are analyzed by capillary gas chromatography.

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