Microfluidic Device Imaging
Microfluidic Device Imaging - Related Videos
08:29
Thermal Measurement Techniques in Analytical Microfluidic Devices
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Cited by 6 •
2015
Here, we present three protocols for thermal measurements in microfluidic devices.
04:35
A Microfluidic Device for Real-Time Imaging and Pressure Tracking During Biofilm Formation
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2026
Source: Kurz, D. L., et al. Microfluidic Platform to Study Bioclogging in Porous Media. J. Vis. Exp. (2022)The video demonstrates the use of a microfluidic device to study biofilm formation. A bacterial suspension is introduced into the microchannel, followed by incubation to allow biofilm development. Flow is then resumed, and pressure monitoring along with imaging is used to detect biofilm formation and pore blockage over time.
10:18
Multi-step Variable Height Photolithography for Valved Multilayer Microfluidic Devices
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Cited by 26 •
2017
Multilayer microfluidic devices often involve the fabrication of master molds with complex geometries for functionality. This article presents a complete protocol for multi-step photolithography with valves and variable height features tunable to any application. As a demonstration, we fabricate a microfluidic droplet generator capable of producing hydrogel beads.
02:10
Assessing Bacterial Motility Using a Microfluidic Device
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2025
Source: Scheidweiler, D., et al. Combining Fluidic Devices with Microscopy and Flow Cytometry to Study Microbial Transport in Porous Media Across Spatial Scales. J. Vis. Exp. (2020).This video demonstrates the use of a microfluidic device to quantify bacterial motility through a porous matrix composed of randomly arranged pillars. Fluorescently labeled bacteria are introduced into the device, imaged via microscopy as they move through the porous matrix into the observation channel, and analyzed...
29:02
Cell Capture Using a Microfluidic Device
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Cited by 1 •
2007
FAQs
Channel geometry and microscale fluid handling determine where a sample travels and which chemical or physical environment it encounters. Because small volumes can be controlled precisely, researchers can observe biological responses under defined conditions rather than treating the sample as a single, unstructured population. This control is especially useful when comparing cell behavior, migration, or sorting outcomes.
The imaging approach should match the change being examined: position, morphology, concentration, or activity. Bright-field and fluorescence provide optical views of material in the channels, while time-lapse imaging adds a sequence of observations that can reveal change over time. Selecting among these options helps align image acquisition with the biological question rather than treating every experiment as a static measurement.
Direct observation links a measured image to the behavior occurring inside the device. Changes in cell shape, location, concentration, or activity can be examined while the sample remains within the controlled channel environment. This connection helps investigators study processes that unfold over time and identify responses to changing chemical or physical conditions, rather than relying only on an endpoint measurement.
A basic workflow begins by placing cells, particles, fluids, or reaction components into the device, then directing them through its channel network under controlled conditions. Optical imaging follows, using bright-field, fluorescence, or time-lapse acquisition as appropriate. The resulting images are analyzed for position, morphology, concentration, or activity, producing measurements tied to the sample’s passage through the device.
In biology, this approach is useful when an experiment must examine cell sorting, migration, behavior, or interactions with chemical and physical environments. It also supports observation of biochemical reactions within the device. These use cases benefit from combining controlled fluid handling with imaging, allowing investigators to relate what the sample encounters to the biological or reaction changes that become visible.
Microfluidic device imaging is advantageous when sample volume is limited or when a process is difficult to measure in a conventional laboratory system. The device can work with small volumes while imaging records changes directly in place. That combination can improve experimental efficiency and expose dynamic biological behavior, including movement or interactions, that a single final measurement might not show.